Fibronectin antibody

Catalog No. ABIN113981

The Rabbit Polyclonal anti-Fibronectin antibody has been validated for IHC (p), EIA, IHC (fro) and RIA. It is suitable to detect Fibronectin in samples from Mouse.

Quick Overview for Fibronectin antibody (ABIN113981)

Target: Fibronectin

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Fibronectin antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Radioimmunoassay (RIA)

Product Details anti-Fibronectin Antibody

Cross-Reactivity (Details): Species reactivity (tested):Mouse.

Purification: Affinity Chromatography.

Immunogen: Purified fibronectin from Murine plasma.

Application Details

Application Notes: This antibody can be used on Frozen and Paraffin Embedded Tissues: IFA (1/80), IHC(P)(1/500) After de-waxing the tissue slices they are treated with 0.2 % hyaluronidase (app.300 U/mg) in TBS 15 min at 37 °C. Thereafter non-specific binding is blocked by blockingserum or 3 % BSA in TBS. For peroxidase systems blocking with 1 % peroxide solution in TBSfor 30 min at RT is recommended. Incubation time: IHC(P) 60 min at RT or 2-8 °C over night. Also suitable for ELISA (1/200) and RIA.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 0.5 mL distilled water, dilute further with appropriate antibody diluent.

Concentration: 1.0 mg/mL

Buffer: PBS without preservative or stabilizer.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Prior to reconstitution store at 2-8 °C. Following reconstitution store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Fibronectin

Target: Fibronectin

Background: Fibronectins are glycoproteins, which occur as dimers of two 200 kDa subunits. They have binding domains for bacterial proteins, collagens, heparin-like molecules and fibrin. Cellular fibronectin is widely distributed in the stroma of malignant tumours.Synonyms: CIG, Cold-insoluble globulin, FN1

Gene ID: 10090

UniProt: P11276