Fibronectin antibody

Catalog No. ABIN113992

This Rabbit Polyclonal antibody specifically detects Fibronectin in IF, IHC (p), EIA, IHC (fro) and RIA. It exhibits reactivity toward Human.

Quick Overview for Fibronectin antibody (ABIN113992)

Target: Fibronectin

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Fibronectin antibody is un-conjugated

Application: Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Radioimmunoassay (RIA)

Product Details anti-Fibronectin Antibody

Specificity: This antibody is specific for Fibronectin (100 %). Collagen type I, III, IV and VI < 0.1 % (RIA at 1/10000 dilution). This antibody may be used to identify fibronectin during wound healing, during tumour progression, and tumour invasion respectively.

Purification: Affinity Chromatography.

Immunogen: Purified fibronectin from human plasma

Application Details

Application Notes: Immunofluorescence: 1/80. Immunohistochemistry: 1/200-1/600 for Paraffin Sections, 60 min at RT or 2-8 °C overnight. ELISA (> / = 1/200). RIA. Positive Control: Human skin. Pretreatment: After de-waxing the tissue slices they are treated with 0.2 % hyaluronidase(app.300 U/mg) in TBS 15 min at 37 °C. There after non-specific binding is blocked byblocking serum or 3 % BSA in TBS. For peroxidase systems blocking with 1 % peroxidesolution in TBS for 30 min at RT is recommended.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 0.5 mL distilled water.

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered solution without BSA or preservatives.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Fibronectin

Target: Fibronectin

Background: Fibronectins are glycoproteins, which occur as dimers of two 200 kDa subunits. They have binding domains for bacterial proteins, collagens, heparin-like molecules and fibrin. Cellular fibronectin is widely distributed in the stroma of malignant tumours.Synonyms: CIG, Cold-insoluble globulin, FN1

Gene ID: 2335

NCBI Accession: NP_002017

UniProt: P02751