BMP10 antibody (AA 86-96)

Catalog No. ABIN114067

This Rabbit Polyclonal antibody specifically detects BMP10 in EIA. It exhibits reactivity toward Human.

Quick Overview for BMP10 antibody (AA 86-96) (ABIN114067)

Target: BMP10 (Bone Morphogenetic Protein 10 (BMP10))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BMP10 antibody is un-conjugated

Application: Enzyme Immunoassay (EIA)

Product Details anti-BMP10 Antibody

Binding Specificity: AA 86-96

Specificity: Bone Morphogenetic Protein 10 (BMP-10) 86-96.

Purification: Ammonium sulphate fractionation

Immunogen: KLH conjugate, sequence: C-L-D-K-G-V-V-T-Y-K-F-K

Application Details

Application Notes: Peptide-ELISA: 1/50 - 1/200. In a simple ELISA using 1 g of antigen coated per well, the50 % OD was observed at an antibody dilution of 1/100. With 1 g of peptide-BSA coatedper well the 50 % OD was observed at 1/2500. Under the same conditions the end pointwas observed at 1/80K. Competitive ELISA: pep-BSA plates, coated at 1 g/well, antibodydiluted 1/10K. Pep-BSA displaces in the range 0.1 to 100 μg/mL. The free peptide does notdisplace in this assay system.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.8 mg/mL (OD280)

Buffer: PBS, pH 7.2, with 0.09 % sodium azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Prior to reconstitution store at 2-8 °C. Following reconstitution store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for BMP10

Target: BMP10 (Bone Morphogenetic Protein 10 (BMP10))

Alternative Name: BMP10

Background: Bone morphogenetic protein-10 (BMP10) belongs to the transforming growth factor beta (TGFß) family. BMPs, named for their initial biological activity of inducing ectopic bone formation, have been linked to multiple steps of cardiac development. BMP10 is translated as a precursor protein (preproprotein) with N-terminal hydrophobic leader peptide followed by prodomain and the mature C-terminal region that is released after the cleavage at multibasic motif RXRR. Data strongly suggest that BMP10 is an essential component in modulating cardiomyocyte proliferation and maturation during cardiac ventricular development.Synonyms: BMP-10, Bone morphogenetic protein 10

Gene ID: 27302

NCBI Accession: NP_055297

UniProt: O95393

Pathways: Hormone Activity, Regulation of Muscle Cell Differentiation