G Protein alpha 0 (AA 345-354) antibody

Catalog No. ABIN114078

Product Details

Target: G Protein alpha 0

Binding Specificity: AA 345-354

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB)

Sequence: {C-}A-N-N-L-R-G-C-G-L-Y

Specificity: This antibody detects G Protein-alpha 0.

Purification: Ammonium Sulphate fractionation

Immunogen: Synthetic peptide corresponding to the amino acids 345 - 354 of the native molecule conjugated to Keyhole Limpet Haemocyanin

Application Details

Application Notes: ELISA (1/100K - 1/400K). Western blot (gives a band at 39 kDa when used under reducing conditions against ratbrain).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 2.4 mg/mL

Buffer: PBS , pH 7.2, 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody after reconstitution at 2-8 °C for one year or (in aliquots) at -20 °C for longer.

Expiry Date: 12 months

Target Details

Target: G Protein alpha 0

Background: GTP binding proteins (G proteins) play a critical role in signal transduction by coupling receptors to effectors. The intracellular consequences of G protein activation include second messenger generation, protein phosphorylation, ion channel activation, gene induction, cell growth, and differentiation. Go belongs to the pertussis toxin sensitive G proteins. It consists of two subtypes of a subunits (ao1 and ao2) derived from alternative splicing of a single gene. ao1 differs from ao2 within the C terminal.Synonyms: Gna0, Gnao, Gnao1, Guanine nucleotide-binding protein G(o) subunit alpha

Gene ID: 14681

NCBI Accession: NP_001106855

UniProt: P18872