Neutrophils antibody (PE)

Catalog No. ABIN114284

The Rat Monoclonal anti-Neutrophils antibody is suitable to detect Neutrophils in samples from Mouse. It has been validated for FACS.

Quick Overview for Neutrophils antibody (PE) (ABIN114284)

Target: Neutrophils

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This Neutrophils antibody is conjugated to PE

Application: Flow Cytometry (FACS)

Clone: 07-04-14

Product Details anti-Neutrophils Antibody (PE)

Characteristics: Absorption / Emission: 488 nm / 575 nm

Purification: Protein A affinity purified Ig

Immunogen: Cultured bone marrow cells

Isotype: IgG2a

Application Details

Application Notes: Flow cytometry (see protocol).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protocol: FLOW CYTOMETRY ANALYSIS: Method: 1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cellpopulation using an NH4Cl lysing buffer.

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: PBS with 0.09 % NaN3 as preservative and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/mL

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Prior to reconstitution store at -70 °C. Following reconstitution store the antibody (in aliquots) at -20 °C for 6 month.

Expiry Date: 6 months

Target Details for Neutrophils

Target: Neutrophils