SAA antibody

Catalog No. ABIN114511

This Mouse Monoclonal antibody specifically detects SAA in WB, EIA and ELISA (Capture). It exhibits reactivity toward Human.

Quick Overview for SAA antibody (ABIN114511)

Target: SAA (Serum Amyloid A (SAA))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This SAA antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA), ELISA (Capture)

Clone: 115

Product Details anti-SAA Antibody

Specificity: This monoclonal antibody reacts with natural and recombinant SAA. Does not show any cross-reaction with other human cytokines or growth factors tested such as IL-1 beta, IL-8, MCAF, TGF-beta and EGF.

Purification: Affinity Chromatography on Protein G

Immunogen: Highly purified recombinant human SAA (MW: 12 kDa)

Isotype: IgG1

Application Details

Application Notes: ELISA: This antibody can be used as a capture antibody in sandwich ELISA applications forhuman SAA detection in combination with a monoclonal tracer/detection antibody(HRPconjugated, Cat. No. : ABIN114514). Suggested Capture Coating Dose: 0.3 g/well, Substrate: TMB. If the above suggestedconditions are followed, approximately 1.1 ng/mL of SAA in serum/plasma or 0.6 ng/mL ofSAA in medium can be detected with an assay range of 0 to 80 ng/mL. Western Blot: An antibody concentration of 0.1-0.5 g will allow visualization of 100 ng/lane of recombinant human SAA.

Restrictions: For Research Use only

Handling

Reconstitution: Double distllated water is recommended to adjust the final concentration to 1.0 mg/mL.

Buffer: 0.01 M PBS, pH 7.2

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Target Details for SAA

Target: SAA (Serum Amyloid A (SAA))

Alternative Name: Serum Amyloid A Protein (SAA)

Background: The serum amyloid A (SAA) family comprises a number of differentially expressed lipoproteins, acute phase SAA1 and SAA2, the former being a major component in plasma, and constitutive SAA's (C-SAAs). Although the liver is the primary site of synthesis of both SAA types, extrhepatic production has been reported. The in vivo concentrations increase by as much as 1000 fold during inflammation. Several studies have expressed it's importance in the diagnosis and monitoring of various diseases. Pathological SAA values are often detected in association with normal CRP concentrations. SAA rises earlier and more sharply than CRP. SAA enhances the binding of HDL's to macrophages and thus helps the delivery of lipid to sites of injury for use in tissue repair. It is thus thought to be an integral part of the disease process. In addition, recent experiments suggest that SAA may play a "houekeeping" role in normal human tissues. Elevated levels of SAA over time predispose secondary amyloidosis, extracellular accumulation of amyloid fibrils, derived from a circulating precursor, in various tissues and organs. The most common form of amyloidosis occurs secondary to chronic inflammatory disease, particularly rheumatoid artheritis.Synonyms: SAA1, SAA2

Gene ID: 6289, 9606

UniProt: P02735