IL16 antibody

Catalog No. ABIN114536

The Mouse Monoclonal anti-IL16 antibody has been validated for WB and EIA. It is suitable to detect IL16 in samples from Human.

Quick Overview for IL16 antibody (ABIN114536)

Target: IL16 (Interleukin 16 (IL16))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This IL16 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Clone: 398

Product Details anti-IL16 Antibody

Specificity: Reactive with natural and recombinant Human IL-16. This monoclonal antibody exhibits no detectable cross-reacting with Human Albumin Serum, Bovine Albumin Serum and other Human cytokines or growth factors tested such as IL-1beta, IL-8, IL-7 MCAF, TGF-beta1, EGF, bFGF, GM-CSF, M-CSF, TNF-alpha.

Purification: Protein G affinity purified

Immunogen: Purified recombinant human IL-16

Isotype: IgG1

Application Details

Application Notes: ELISA: This antibody can be used as a capture antibody in sandwich ELISA applications forhuman IL-16 detection in combination with a monoclonal tracer/detection antibody. Suggested Capture Coating Dose: 0.3g/well, Substrate: TMB. If the above suggested conditions are followed, approximately 20pg/mL of IL-16 inserum/plasma or 15 pg/mL of IL-16 in cell medium/urine can be detected with an assayrange of 0-1000 pg/mL
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with Double distillated water is recommended and to adjust the final concentration to 1.0 mg/mL

Buffer: 0.01 M PBS, pH 7.2

Storage: 4 °C

Storage Comment: Store the antibody at 2 - 8 °C. Do not freeze.

Target Details for IL16

Target: IL16 (Interleukin 16 (IL16))

Alternative Name: Interleukin-16 / IL16

Background: IL16 is a proinflammatory cytokine that is chemotactic for CD4+ T lymphocytes, monocytes, and eosinophils. IL16 is synthesized as a precursor molecule (pro IL16) of approximately 68 kDa and 631 amino acid residues lacking a signal peptide. The sequence and structure of IL16 is conserved across species. In addition to inducing chemotaxis, IL16 upregulates the IL2 receptor and upregulates HLADR expression. It also inhibits T cell receptor (TCR)/CD3 dependent activation, and suppresses HIV1 replication in vitro. Sources of IL16 include epithelial cells, mast cells, T lymphocytes (CD4+ and CD8+), macrophages, synovial fibroblasts, and eosinophils.Synonyms: IL-16, LCF, Lymphocyte chemoattractant factor

Gene ID: 3603

NCBI Accession: NP_001165599

UniProt: Q14005