CDK1 antibody

Catalog No. ABIN114583

This Mouse Monoclonal antibody specifically detects CDK1 in WB, IF, IP and IHC (p). It exhibits reactivity toward Human and Cow.It has been mentioned in 1 publication

Quick Overview for CDK1 antibody (ABIN114583)

Target: CDK1 (Cyclin-Dependent Kinase 1 (CDK1))

Reactivity: Human, Cow

Host: Mouse

Clonality: Monoclonal

Conjugate: This CDK1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: POH-1

Product Details anti-CDK1 Antibody

Specificity: The antibody POH-1 specifically detects Cdk1 (p34Cdc2). Staining of Cdk1 reflects the proliferating potential of respective tissue.

Cross-Reactivity (Details): Species reactivity (tested):Human, Non-Human Primates, Bovine.

Purification: Affinity Chromatography on Protein A.

Immunogen: Recombinant human cdc2 protein.

Isotype: IgG2a

Application Details

Application Notes: Western Blot. Immunoprecipitation. Immunocytochemistry. Immunohistochemistry on Paraffin Sections: 10 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH ~7.4, 15 mM Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-CDK1 antibody (ABIN114583)

Jaeger, Koczan, Thiesen, Ibrahim, Gross, Spang, Kunz: "Gene expression signatures for tumor progression, tumor subtype, and tumor thickness in laser-microdissected melanoma tissues." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 13, Issue 3, pp. 806-15, (2007) (PubMed).

Target Details for CDK1

Target: CDK1 (Cyclin-Dependent Kinase 1 (CDK1))

Alternative Name: CDK1

Background: The p34cdc2 is a 34 kDa phosphoprotein with protein kinase activity that functions in the G2/M phase transition of the cell cycle. Expression of p34cdc2 reflects the proliferating potential of positively staining tissue. The maximal activity of p34cdc2 is achieved when it is fully dephosphorylated. It is a 34kD catalytic subunit of maturation promoting factor (MPF) and complexes with both cyclin A and cyclin B in mammalian cells.Synonyms: CDC2, CDC28A, CDK1, CDKN1, Cell division control protein 2 homolog, Cell division protein kinase 1, Cyclin-dependent kinase 1, P34CDC2, p34 protein kinase

Gene ID: 983

NCBI Accession: NP_001777

UniProt: P06493

Pathways: Cell Division Cycle, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Toll-Like Receptors Cascades, Synthesis of DNA