ACP5 antibody

Catalog No. ABIN114707

The Mouse Monoclonal anti-ACP5 antibody is suitable to detect ACP5 in samples from Human. It has been validated for EIA.

Quick Overview for ACP5 antibody (ABIN114707)

Target: ACP5 (Acid Phosphatase 5, Tartrate Resistant (ACP5))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ACP5 antibody is un-conjugated

Application: Enzyme Immunoassay (EIA)

Clone: TRAP-01

Product Details anti-ACP5 Antibody

Specificity: The Anti Human TRAP 5 Antibody, Clone: TRAP-01 is a mouse monoclonal antibody against TRAP 5.

Purification: Affinity chromatography on protein G

Immunogen: Recombinant human TRAP 5 expressed in Sf 9 cells.

Isotype: IgG1

Application Details

Application Notes: Enzyme Immunoassay.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 1 mL of deionized water and let the lyophilized pellet dissolve completely. Slight turbidity may occur after reconstitution, which does not affect activity of the antibody. In this case clarify the solution by centrifugation.

Concentration: 1.0 mg/mL

Buffer: 0.05 M phosphate buffer, 0.1 M NaCl, pH 7.2. AZIDE FREE, None

Preservative: Azide free

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Prior to reconstitution store at -70 °C. Following reconstitution store the antibody (in aliquots) at -20 °C for 6 month.

Expiry Date: 6 months

Target Details for ACP5

Target: ACP5 (Acid Phosphatase 5, Tartrate Resistant (ACP5))

Alternative Name: ACP5 / TR-AP

Background: TRAP 5 (serum band 5 tartrate-resistant acid phosphatase, TRACP 5, EC 3.1.3.2) is a glycoprotein of 35-37 kDa. TRAP 5 belongs to the most abundant enzymes in osteoclasts. It is expressed in certain differentiated cells of the mononuclear phagocyte system, particularly osteoclasts and alveolar macrophages, where it takes an active part in bone resorption process. High blood levels of TRAP 5 are usually associated with active bone remodelling. Increased serum levels are observed during normal bone growth among healthy children. Elevated serum TRAP levels have been detected in diseases characterized by increased bone resorption, Pagets disease of the bone, hemodialysis, primary hyperparathyroidism, metastatic malignancies involving bone resorption, multiple myeloma and bilaterally ovariectomized women. Post-menopausal women have higher levels of serum than post-menopausal women on estrogen replacement therapy. Therefore specific determination of TRAP 5 activity can be essential for clinical assessment of bone metabolism.Synonyms: Acid phosphatase 5 tartrate resistant, TRAP5, TRAcP, Tartrate-resistant acid ATPase, Tartrate-resistant acid phosphatase type 5

Gene ID: 54

NCBI Accession: NP_001104504

UniProt: P13686

Pathways: Transition Metal Ion Homeostasis