GFAP antibody

Catalog No. ABIN115304

The Rabbit Polyclonal anti-GFAP antibody is suitable to detect GFAP in samples from Human, Rat, Mouse, Cow, Chicken, Guinea Pig and Cat. It has been validated for IHC (p).

Quick Overview for GFAP antibody (ABIN115304)

Target: GFAP (Glial Fibrillary Acidic Protein (GFAP))

Reactivity: Human, Rat, Mouse, Cow, Chicken, Guinea Pig, Cat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GFAP antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-GFAP Antibody

Specificity: This antibody reacts with GFAP. In the central nervous system, this antibody stains Astrocytes and some groups of Ependymal cells. However, in the peripheral nervous system, it stains Schwann cells, satellite cells and enteric glial cells. Cellular localization: Cytoplasmic.

Purification: Purified

Immunogen: Glial fibrillary protein isolated from cow spinal cord.

Application Details

Application Notes: Immunohistochemistry on Formalin-Fixed Paraffin Embedded Sections: This antibody maybe diluted to a titer of 1/50-1/250 in an ABC method (30 minutes at room temperature). Recommended Positive Control: Brain.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.

Target Details for GFAP

Target: GFAP (Glial Fibrillary Acidic Protein (GFAP))

Alternative Name: GFAP

Background: Glial fibrillary acidic protein (GFAP) is a member of the class III intermediate filament protein family. It is heavily, and specifically, expressed in astrocytes and certain other astroglia in the central nervous system, in satellite cells in peripheral ganglia, and in non myelinating Schwann cells in peripheral nerves. In addition, neural stem cells frequently strongly express GFAP. Antibodies to GFAP are therefore very useful as markers of astrocytic cells. In addition many types of brain tumor, presumably derived from astrocytic cells, heavily express GFAP. GFAP is also found in the lens epithelium, Kupffer cells of the liver, in some cells in salivary tumors and has been reported in erythrocytes.Synonyms: Glial Fibrillary Acidic Protein

Gene ID: 2670

NCBI Accession: NP_001229305

UniProt: P14136