AMACR antibody

Catalog No. ABIN115447

This Rabbit Polyclonal antibody specifically detects AMACR in IHC (p) and IHC (fro). It exhibits reactivity toward Human.

Quick Overview for AMACR antibody (ABIN115447)

Target: AMACR (alpha-Methylacyl-CoA Racemase (AMACR))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AMACR antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-AMACR Antibody

Specificity: Reacts to P504S Alpha-Methylacyl-CoA Racemace.
Cellular Localization: Cytoplasmic.

Purification: Purified

Immunogen: A synthetic human AMACR peptide.

Application Details

Application Notes: Immunohistochemistry on Formalin-Fixed Paraffin Embedded Sections: 1/25-1/50 in anABC method (30 minutes at room temperature). Formalin fixed paraffin embedded tissue sections require high temperature antigenunmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Recommended Positive Control: Prostate carcinoma.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for AMACR

Target: AMACR (alpha-Methylacyl-CoA Racemase (AMACR))

Alternative Name: AMACR / RACE

Background: P504S (AMARC) has been recently described as prostate cancer-specific gene that encodes a protein involved in the beta-oxidation of branched chain fatty acids. Expression of P504S protein is found in prostatic adenocarcinoma but not in benign prostatic tissue. It stains premalignant lesions of prostate: high-grade prostatic intraepithelial neoplasia (PIN) and atypical adenomatous hyperplasia. P504S can be used as a positive marker for PIN.Synonyms: 2-methylacyl-CoA racemase, Alpha-methylacyl-CoA racemase, P504S

Gene ID: 23600

NCBI Accession: NP_055139

UniProt: Q9UHK6

Pathways: Monocarboxylic Acid Catabolic Process