HBxAg antibody

Catalog No. ABIN115505

Product Details anti-HBxAg Antibody

Target: HBxAg (Hepatitis B Virus X Antigen (HBxAg))

Reactivity: Hepatitis B Virus (HBV)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HBxAg antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA), Immunoprecipitation (IP)

Sequence: Total 154 AA: 153 AA of recombinan t HBx protein and one extra AA, N-terminal methionin (underlined ). MAARVCCQLD PARDVLCLRP VGAESRGRPV SGPFGTLPSP SSSAVPADHG AHLSLRGLPV CAFSSAGPCA LRFTSARRME TTVNAHQVLP KVLHKRTLGL SAMSTTDLEA YFKDCLFKDW EELGEEIRLK VFVLGGCRHK LVCSPAPCNF FTSA

Purification: Immunoaffinity Chromatography on a column with immobilized recombinant Hepatitis B Protein X

Immunogen: Recombinant Hepatitis B Protein X from E. coli.

Application Details

Application Notes: ELISA. (Titer is defined by Indirect ELISA, it is: >1: 100,000 for antibody concentration 1 mg/mL, 25 ng of antigen are coated per well, and is then defined at a point of maximal decrease of thetitration curve). Western blotting. Immunoprecipitaion.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Add 0.05 mL of deionized water and let the lyophilized pellet dissolve completely. Slight turbidity may occur after reconstitution, which does not affect activity of the antibody. In this case clarify the solution by centrifugation.

Buffer: 0.05 M Phosphate buffer, 0.5 M NaCl, pH 7.2 without preservatives

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Stable the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-HBxAg antibody (ABIN115505)

Gouas, Shi, Hautefeuille, Ortiz-Cuaran, Legros, Szymanska, Galy, Egevad, Abedi-Ardekani, Wiman, Hantz, Caron de Fromentel, Chemin, Hainaut: "Effects of the TP53 p.R249S mutant on proliferation and clonogenic properties in human hepatocellular carcinoma cell lines: interaction with hepatitis B virus X protein." in: Carcinogenesis, Vol. 31, Issue 8, pp. 1475-82, (2010) (PubMed).

Zhang, Yang, Zhang, Xu, Liao, Song, Wang: "Induction of hepatic enzymes and oxidative stress in Chinese rare minnow (Gobiocypris rarus) exposed to waterborne hexabromocyclododecane (HBCDD)." in: Aquatic toxicology (Amsterdam, Netherlands), Vol. 86, Issue 1, pp. 4-11, (2008) (PubMed).

Target Details for HBxAg

Target: HBxAg (Hepatitis B Virus X Antigen (HBxAg))

Alternative Name: Hepatitis B X Protein / HBx (HBxAg Products)

Target Type: Viral Protein

Background: The recombinant Hepatitis B Protein X is 100 % homologous with the natural Hepatitis B Protein X. Hepatitis B virus X protein (HBx) is a 17 kD transcriptional coactivator that plays a significant role in the regulation of genes involved in inflammation and cell survival. It regulates many transcription factors including nuclear factor kappa B (NF-kappaB) and plays a key role in hepatocarcinogenesis. HBx facilitates the binding of cAMP response element binding protein (CREB) to its responsive element. HBx stabilizes the cellular coactivator ASC-2 through direct protein-protein interaction, affecting the regulation of genes actively transcribed in liver cancer cells. HBx transactivates both JNK and MAPK signal transduction pathways in association with the mobilization of cytosolic Ca2+. The communication between HBx and general transcription factor TFIIB is also one of the mechanisms which account for its transcriptional transactivation. HBx decreased the expression of PTEN a known tumor suppressor and a negative regulator of phosphatidylinositol 3'-kinase/AKT and HBx decreased the expression of PTEN in HBx-transfected cells. The etiology of hepatocellular carcinoma (HCC) is involved with hepatitis B virus (HBV) infection and HBx in particular plays a role in the development of HBV-related HCC. The persistence of HBx is important to the pathogenesis of early HCC and HBx expression in the liver during chronic HBV infection may be an important prognostic marker for the development of HCC.Synonyms: HBV Protein X

Molecular Weight: 17 kDa