LYVE1 antibody (AA 24-231)

Catalog No. ABIN115663

This Rabbit Polyclonal antibody specifically detects LYVE1 in WB, FACS, IHC (p), EIA and IHC (fro). It exhibits reactivity toward Human.

Quick Overview for LYVE1 antibody (AA 24-231) (ABIN115663)

Target: LYVE1 (Lymphatic Vessel Endothelial Hyaluronan Receptor 1 (LYVE1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LYVE1 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-LYVE1 Antibody

Binding Specificity: AA 24-231

Specificity: This antibody will detect LYVE-1 on the surface of lymphatic endothelial cells by Immunohistochemistry. It detects a 70 kDa LYVE-1 band in Western blotting. This antibody is not reactive with Mouse LYVE-1.

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Antigen Affinity Chromatography

Immunogen: Highly pure recombinant Human soluble LYVE-1 produced in insect cells. It consists of amino acid 24 (Ser) to 232 (Gly) and is fused to a C-terminal His-tag (6xHis).

Application Details

Application Notes: Immunohistochemistry on Frozen and Paraffin Embedded Sections (1-5 μg/mL). Heat mediated antigen retrieval is recommended when staining paraffin embeddedsections. Western blot (1-2 μg/mL). ELISA (1-15 μg/mL). Flow Cytometry (3-20 μg/mL).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/mL

Buffer: 5 mM PBS, pH 7.2 without preservatives or stabilizers

Preservative: Without preservative

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C. Protect from Light!

Target Details for LYVE1

Target: LYVE1 (Lymphatic Vessel Endothelial Hyaluronan Receptor 1 (LYVE1))

Alternative Name: LYVE-1

Background: LYVE-1 has been identified as a major receptor for HA (extracellular matrix glycosamino-glycan hyaluronan) on the lymph vessel wall. The deduced amino acid sequence of LYVE-1 predicts a 322-residue type I integral membrane polypeptide 41 % similar to the CD44 HA receptor with a 212-residue extracellular domain containing a single Link module the prototypic HA binding domain of the Link protein superfamily. Like CD44, the LYVE-1 Molecule binds both soluble and immobilized HA. However, unlike CD44, the LYVE-1 Molecule colocalizes with HA on the luminal face of the lymph vessel wall and is completely absent from blood vessels. Hence, LYVE-1 is the first lymph-specific HA receptor to be characterized and is a uniquely powerful marker for lymph vessels.Synonyms: CRSBP-1, CRSBP1, Cell surface retention sequence-binding protein 1, Extracellular link domain-containing protein 1, HAR, Hyaluronic acid receptor, LYVE1, Lymphatic vessel endothelial hyaluronic acid receptor 1, XLKD1

Gene ID: 10894

NCBI Accession: NP_006682

UniProt: Q9Y5Y7

Pathways: Glycosaminoglycan Metabolic Process