Podoplanin antibody

Catalog No. ABIN115686

Cited in 1 publication. The Rabbit Polyclonal anti-Podoplanin antibody (ABIN115686) specifically detects Podoplanin in WB, IF and IP. The antibody is reactive with Human samples.

Quick Overview for Podoplanin antibody (ABIN115686)

Target: Podoplanin (PDPN)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Podoplanin antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP)

Product Details anti-Podoplanin Antibody

Specificity: This antibody detects Podoplanin. The antibody is not reactive with mouse podoplanin.

Purification: Protein A affinity chromatography

Immunogen: Recombinant ectodomain of human gp36 (Podoplanin)

Application Details

Application Notes: Immunoprecipitation: 2-4 μg/sample. Immunofluorescence: 1-2 μg. Western blotting: 1-2 μg/mL).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile water to a concentration of > 0.5 mg/mL.

Buffer: PBS, pH 7.4, without preservative or stabilizer

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: The lyophilized IgG is stable at 2-8 °C for one month and for one year from despatch when kept at -20 °C. When reconstituted in sterile water to a concentration of > 0.5 mg/ml the antibody is stable for six weeks at 2-8 °C in the presence of a preservative. Reconstituted antibody can also be aliquotted and stored at -20 °C to -70 °C for six months without detectable loss of activity.

Expiry Date: 12 months

References for anti-Podoplanin antibody (ABIN115686)

Conrad, Niess, Huss, Huber, von Luettichau, Nelson, Ott, Jauch, Bruns: "Multipotent mesenchymal stem cells acquire a lymphendothelial phenotype and enhance lymphatic regeneration in vivo." in: Circulation, Vol. 119, Issue 2, pp. 281-9, (2009) (PubMed).

Target Details for Podoplanin

Target: Podoplanin (PDPN)

Alternative Name: Podoplanin

Background: Podoplanin is a highly O-glycosylated integral membrane protein that is specifically expressed in the endothelium of lymphatic capillaries but not in the blood vasculature. In normal skin and kidney, Podoplanin colocalized with VEGFR-3/FLT4, another marker for lymphatic endothelial cells.Synonyms: Aggrus, GP36, Glycoprotein 36, PA2.26 antigen, PDPN, PSEC0003, PSEC0025, T1-alpha

Gene ID: 10630

NCBI Accession: NP_001006625

UniProt: Q86YL7

Pathways: Dicarboxylic Acid Transport