PROX1 antibody (C-Term)

Catalog No. ABIN115695

This anti-PROX1 antibody is a Rabbit Polyclonal antibody detecting PROX1 in WB, IF, IHC (p), EIA and IHC (fro). Suitable for Human, Mouse and Rat.

Quick Overview for PROX1 antibody (C-Term) (ABIN115695)

Target: PROX1 (Prospero Homeobox 1 (PROX1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PROX1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-PROX1 Antibody

Binding Specificity: C-Term

Specificity: This antibody will detect Prox-1 in the nucleus of lymphatic endothelial cells by Immunostaining. This antibody cross reacts with Equine Prox-1 in Immunohistochemistry using Paraffin-Embedded Sections (Figure 2).

Purification: Antigen-Affinity Chromatography.

Immunogen: Recombinant Human C-terminal part of the Homeobox Transcription Factor Prox-1 (homeo domain and prospero domain).

Application Details

Application Notes: Immunofluorescence: Use 1/750-1/1000 dilution. Western Bloting: Use 1-5 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with distilled sterile water to a concentration of 0.1-1.0 mg/mL

Buffer: 5 mM PBS, pH 7.2 without preservatives

Preservative: Without preservative

Handling Advice: Dilute only prior to immediate use

Storage: 4 °C

Storage Comment: Store the antibody at 2-8 °C as an undiluted liquid. DO NOT FREEZE! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.

Target Details for PROX1

Target: PROX1 (Prospero Homeobox 1 (PROX1))

Alternative Name: PROX-1

Background: Prox-1 is a homeobox gene and acts as a master switch for lymphatic endothelial phenotype. Expression of Prox-1 in blood endothelial cells induces expression of other lymphatic marker genes. Together with Podoplanin, Prox-1 can be used to reliably distinguish lymphatic vessels from blood vessels. Prox-1 is expressed in CNS, eye, pancreas, liver and heart, and it is one of the most specific and reliable markers for lymphatic endothelial cells.Synonyms: Homeobox prospero-like protein PROX1, PROX1, Prospero homeobox protein 1

Gene ID: 5629

NCBI Accession: NP_002754

UniProt: Q92786

Pathways: Stem Cell Maintenance, Regulation of Muscle Cell Differentiation