IL-8 antibody

Catalog No. ABIN115766

The Mouse Monoclonal anti-IL-8 antibody has been validated for EIA and IHC (fro). It is suitable to detect IL-8 in samples from Human.

Quick Overview for IL-8 antibody (ABIN115766)

Target: IL-8 (IL8) (Interleukin 8 (IL8))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This IL-8 antibody is un-conjugated

Application: Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: SB-84-8

Product Details anti-IL-8 Antibody

Purification: Protein G Affinity Chromatography

Immunogen: Highly purified recombinant Human IL-8

Isotype: IgG1

Application Details

Application Notes: ELISA: In a Sandwich ELISA (assuming 100 μL/well), a concentration of 2.0-4.0 μg/mL ofthis antibody will detect at least 1000 pg/mL of recombinant Human IL-8 when used withbiotinylated antigen affinity purified anti-Human IL-8 (cat. no. PP1030B) as the detectionantibody at a concentration of approximately 0.5-1.0 μg/mL. Western Blot: To detect Human IL-8, this antibody can be used at a concentration of0.20-0.40 μg/mL. Used in conjunction with compatible secondary reagents the detectionlimit for recombinant hIL-8 is 2.0-4.0 ng/lane, under non-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile water to a concentration of 1.0 mg/mL.

Buffer: PBS without preservatives

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: The lyophilized antibody is stable at room temperature for one month and at -20 °C for one year from despatch. The reconstituted antibody is stable for two weeks at 2-8 °C. Frozen aliquots are stable for six months when stored at -20 °C.

Expiry Date: 12 months

Target Details for IL-8

Target: IL-8 (IL8) (Interleukin 8 (IL8))

Alternative Name: Interleukin-8 / IL8

Background: IL8 is a member of the CXC chemokine family. This family of small basic heparan-binding proteins are proinflammatory and primarily mediate the activation and migration of neutrophils into tissue from peripheral blood. This chemokine is one of the major mediators of the inflammatory response and is secreted by several cell types in response to an inflammatory stimulus. It functions as a chemoattractant, and is also a potent angiogenic factor. IL8 attracts neutrophils, basophils, and T-cells, but not monocytes. Cystic fibrosis (CF) is characterized by severe lung inflammation. The inflammatory process is believed to be caused by massive overproduction of the proinflammatory protein IL8, and the high levels of IL8 in the CF lung are therefore believed to be the central mechanism behind CF lung pathophysiology.Synonyms: CXCL8, Emoctakin, GCP1, Granulocyte chemotactic protein 1, MDNCF, MONAP, Monocyte-derived neutrophil chemotactic factor, Monocyte-derived neutrophil-activating peptide, NAP1, Neutrophil-activating protein 1, Protein 3-10C, T-cell chemotactic factor

Gene ID: 9606

UniProt: P10145

Pathways: TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of G-Protein Coupled Receptor Protein Signaling, ER-Nucleus Signaling, Hepatitis C, Autophagy