CCL3 antibody

Catalog No. ABIN115769

The Mouse Monoclonal anti-CCL3 antibody has been validated for WB, EIA, Func and IHC (fro). It is suitable to detect CCL3 in samples from Human.

Quick Overview for CCL3 antibody (ABIN115769)

Target: CCL3 (Chemokine (C-C Motif) Ligand 3 (CCL3))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CCL3 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA), Functional Studies (Func), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-CCL3 Antibody

Specificity: Recognizes Human Macrophage Inflammatory Protein 1 alpha (MIP1-alpha). Other species not tested.

Purification: Affinity Chromatography on Protein G

Immunogen: Highly purified recombinant Human MIP-1 alpha.

Isotype: IgG1

Application Details

Application Notes: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofHuman MIP1-alpha (100 ng/mL), a concentration of 5.0-10.0 μg/mL of this antibody isrequired. ELISA: In a Sandwich ELISA (assuming 100 μL/well), a concentration of 2.0-4.0 μg/mL ofthis antibody will detect at least 2000 pg/mL of recombinant Human MIP1-alpha when usedwith Biotin anti-Human MIP1-alpha antibody (cat. PP1050B) as the detection antibody at aconcentration of approximately 1.0-2.0 μg/mL. Western Blot: To detect HUman MIP1-alpha by Western Blot analysis this antibody can beused at a concentration of 2.0-4.0 μg/mL. Used in conjunction with compatible secondaryreagents the detection limit for recombinant Human MIP1-alpha is 1.0-2.0 ng/lane, undernon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile water to a concentration of 1.0 mg/mL.

Buffer: PBS without preservatives

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody at -20 °C.

Target Details for CCL3

Target: CCL3 (Chemokine (C-C Motif) Ligand 3 (CCL3))

Alternative Name: MIP1 alpha / CCL3

Background: Macrophage Inflammatory Protein 1 alpha (MIP1 alpha) and MIP1 beta, two closely related but distinct proteins, were originally co-purified from medium conditioned by a LPS-stimulated murine macrophage cell line. Mature mouse MIP1 alpha shares approximately 77 % and 70 % amino acid identity with human MIP1 alpha and mouse MIP1 beta, respectively. MIP1 proteins are expressed primarily in T cells, B cells, and monocytes after antigen or mitogen stimulation. Has adhesive effects on lymphocytes. MIP1 alpha can inhibit the proliferation of hematopoietic stem cells in vitro as well as in vivo. A signal transducing receptor, designated the C-C chemokine receptor-1 (C-C CKR-1) with seven transmembrane domains that bind MIP1 alpha, MIP1 beta, MCP1 and RANTES with varying affinities, has been isolated.Synonyms: C-C motif chemokine 3, CCL-3, G0/G1 switch regulatory protein 19-1, G0S19-1 protein, LD78 alpha, MIP-1-alpha, MIP1A, Macrophage Inflammatory Protein 1 alpha, SCYA3, Small-inducible cytokine A3, Tonsillar lymphocyte LD78 alpha protein

Gene ID: 6348

NCBI Accession: NP_002974

UniProt: P10147

Pathways: Cellular Response to Molecule of Bacterial Origin, Autophagy