FGF9 antibody (Biotin)

Catalog No. ABIN115847

The Rabbit Polyclonal anti-FGF9 antibody is suitable to detect FGF9 in samples from Mouse. It has been validated for WB and EIA.

Quick Overview for FGF9 antibody (Biotin) (ABIN115847)

Target: FGF9 (FGF-9) (Fibroblast Growth Factor 9 (FGF-9))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FGF9 antibody is conjugated to Biotin

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-FGF9 Antibody (Biotin)

Specificity: Recognises Fibroblast Growth Factor 9 (FGF-9).

Purification: Affinity chromatography.

Immunogen: Highly pure (>98%) recombinant mFGF-9.

Application Details

Application Notes: ELISA: (Direct): To detect mFGF-9 by direct ELISA (using 100 μL/well antibody solution) aconcentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylated polyclonalantibody, in conjunction with compatible secondary reagents, allows the detection of atleast 0.2- 0.4 ng/well of recombinant mFGF-9. (Sandwich): To detect mFGF-9 by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylated polyclonalantibody, in conjunction with Polyclonal Anti-Murine FGF-9 (PP002P) as a captureantibody, allows the detection of at least 0.2-0.4 ng/well of recombinant mFGF-9. Western Blot: To detect mFGF-9 by Western Blot analysis this antibody can be used at a concentration of0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limitfor recombinant mFGF-9 is 1.5-3.0 ng/lane, under either reducing or non-reducingconditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Target Details for FGF9

Target: FGF9 (FGF-9) (Fibroblast Growth Factor 9 (FGF-9))

Alternative Name: FGF9

Background: FGF9 is a heparin binding growth factor, which is a member of the FGF family of proteins. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. FGF9 is produced mainly by neurons and may have a role in glial cell growth and differentiation during development, gliosis during repair and regeneration of brain tissue after damage, differentiation and survival of neuronal cells, and growth stimulation of glial tumors.Synonyms: Fibroblast growth factor 9, GAF, Glia-activating factor, HBGF9, Heparin-binding growth factor 9

Gene ID: 14180

NCBI Accession: NP_038546

UniProt: P54130