GM-CSF antibody

Catalog No. ABIN115853

The Rabbit Polyclonal anti-GM-CSF antibody has been validated for WB, EIA and Func. It is suitable to detect GM-CSF in samples from Mouse.

Quick Overview for GM-CSF antibody (ABIN115853)

Target: GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GM-CSF antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA), Functional Studies (Func)

Product Details anti-GM-CSF Antibody

Specificity: This antibody reacts with Mouse GM-CSF.

Purification: Affinity Chromatography employing immobilized mGM-CSF matrix.

Immunogen: E.coli derived Highly pure (>98%) recombinant Murine GM-CSF Remarks: Mol. Weight: ~150 kDa

Application Details

Application Notes: NeutralizationTo yield one-half maximal inhibition [ND50] of the biological activity of mGM-CSF (0.60 ng/mL), a oncentration of 0.012-0.02 μg/mL of this antibody is required. ELISAIndirect: To detect Mouse GM-CSF by indirect ELISA (using 100 μL/well antibody solution) aconcentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with compatible secondary reagents, allows the detection of atleast 0.2-0.4 ng/well of recombinant Mouse GM-CSF. Sandwich: To detect mGM-CSF by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with Biotinylated Anti-Murine GM-CSF (PP003B1 or PP003B2) as adetection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinantmGM-CSF. Western Blot: To detect Mouse GM-CSF by Western Blot analysis this antibody can be used at aconcentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant mGM-CSF is 1.5-3.0 ng/lane, under either reducing ornon-reducing conditions.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Target Details for GM-CSF

Target: GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))

Alternative Name: GM-CSF

Background: The cytokine GM-CSF stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes. Used in myeloid reconstitution following bone marrow transplant, bone marrow transplant engraftment failure or delay, mobilization and following transplantation of autologous peripheral blood progenitor cells, and following induction chemotherapy in older adults with acute myelogenous leukemia.Synonyms: CSF2, Colony stimulating factor 2, GMCSF, Granulocyte-macrophage colony-stimulating factor, Molgramostin, Sargramostim

Gene ID: 12981

NCBI Accession: NP_034099

UniProt: P01587

Pathways: JAK-STAT Signaling, Cellular Response to Molecule of Bacterial Origin