IL-10 antibody (Biotin)

Catalog No. ABIN115867

This Rabbit Polyclonal antibody specifically detects IL-10 in WB and EIA. It exhibits reactivity toward Mouse.

Quick Overview for IL-10 antibody (Biotin) (ABIN115867)

Target: IL-10 (IL10) (Interleukin 10 (IL10))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL-10 antibody is conjugated to Biotin

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-IL-10 Antibody (Biotin)

Specificity: Recognizes Murine IL-10.

Cross-Reactivity (Details): Species reactivity (tested):Mouse.

Purification: Affinity Chromatography.

Immunogen: Highly pure (>98%) recombinant Mouse IL-10.

Application Details

Application Notes: ELISA: To detect mIL-10 by direct ELISA (using 100 ml/well antibody solution) this antibody can beused at a concentration of 0.15-0.30 μg/mL. Used in conjunction with compatiblesecondary reagents, allows the detection of at least 0.2 ng/well of recombinant mIL-10. Western Blot: To detect mIL-10 by Western Blot analysis this antibody can be used at a concentration of0.1-0.2 mg/mL. Used in conjunction with compatible secondary reagents the detection limitfor recombinant mIL-10 is 1.5-3.0 ng/lane, under either reducing or non-reducingconditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing. Centrifuge vial prior to opening.

Storage: 4 °C/-20 °C

Storage Comment: The lyophilized antibody can be stored for one year from despatch at -20 °C. Reconstituted antibody is stable for two weeks at 2-8 °C, frozen aliquots are stable for six months at -20 °C.

Expiry Date: 12 months

Target Details for IL-10

Target: IL-10 (IL10) (Interleukin 10 (IL10))

Alternative Name: Interleukin-10 / IL10

Background: Interleukin 10 (IL-10) is a 178 amino acid protein that is primarily secreted by TH2 clones. IL-10 has dual functions, the first of which is the suppression of cytokine production by TH1 clones responding to antigen presented by monocyte and macrophage antigen presenting cells (APCs). The second function consists of the inhibition of response of cytokine targeted cells, possibly by the downregulation of CD25 (the interleukin-2 receptor) on macrophages and B lymphocytes. IL-10 exerts its biological activity through the IL-10 receptor (IL-10R), a 560 amino acid, 110 kDa glycoprotein whose expression can be induced in cultured macrophages and fibroblasts by elevated in HIV-1 infected individuals and has been implicated in the progression of the disease.Synonyms: CSIF, Cytokine synthesis inhibitory factor, IL-10, TGIF

Gene ID: 16153

NCBI Accession: NP_034678

UniProt: P18893

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location, Cancer Immune Checkpoints