IL-3 antibody

Catalog No. ABIN115877

The Rabbit Polyclonal anti-IL-3 antibody has been validated for WB, EIA and Func. It is suitable to detect IL-3 in samples from Mouse.

Quick Overview for IL-3 antibody (ABIN115877)

Target: IL-3 (Interleukin 3 (IL-3))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL-3 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA), Functional Studies (Func)

Product Details anti-IL-3 Antibody

Specificity: This antibody recognises Mouse Interleukin-3 (IL-3).

Purification: Affinity chromatography.

Immunogen: Highly pure (>98%) recombinant mIL-3

Application Details

Application Notes: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of mIL-3 (0.5 ng/mL), aconcentration of 0.15-0.20 μg/mL of this antibody is required. ELISA: (Indirect): To detect mIL-3 by indirect ELISA (using 100 μL/well antibody solution) aconcentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with compatible secondary reagents, allows the detection of atleast 0.2-0.4 ng/well of recombinant mIL-3. (Sandwich): To detect mIL-3 by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with Biotinylated Anti-Murine IL-3 (PP010B) as a detectionantibody, allows the detection of at least 0.2-0.4 ng/well of recombinant mIL-3. Western Blot: To detect mIL-3 by Western Blot analysis this antibody can be used at a concentration of0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limitfor recombinant mIL-3 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing. Centrifuge vial prior to opening!

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Target Details for IL-3

Target: IL-3 (Interleukin 3 (IL-3))

Alternative Name: Interleukin-3 / IL3

Background: IL3 (Interleukin 3) is a 15 kDa cytokine that is primarily secreted by activated T lymphocytes and stimulates the proliferation and differentiation of hematopoietic cells. IL3 acts in hematopoiesis by controlling the production, differentiation, and function of two related white cell populations of the blood, the granulocytes and the monocytes/macrophages. It induces granulocytes, macrophages, mast cells, stem cells, erythroid cells, eosinophils and megakaryocytes and is expressed by activated T cells, mast cells, and natural killer cells. IL3 not only supports growth of both pluripotent stem cells and the more differentiated committed progenitors, but it also stimulates the functional activity of some fully differentiated cells. IL3 has also been shown to protect mast cells from undergoing apoptosis.Synonyms: Hematopoietic growth factor, IL-3, MCGF, Mast cell growth factor, Multipotential colony-stimulating factor, P-cell-stimulating factor

Gene ID: 16187

NCBI Accession: NP_034686

UniProt: P01586

Pathways: JAK-STAT Signaling, Regulation of Carbohydrate Metabolic Process, Autophagy