CCL7 antibody (Biotin)

Catalog No. ABIN115895

The Goat Polyclonal anti-CCL7 antibody has been validated for WB and EIA. It is suitable to detect CCL7 in samples from Mouse.

Quick Overview for CCL7 antibody (Biotin) (ABIN115895)

Target: CCL7 (Chemokine (C-C Motif) Ligand 7 (CCL7))

Reactivity: Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This CCL7 antibody is conjugated to Biotin

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-CCL7 Antibody (Biotin)

Specificity: Reacts with Macrophage Chemotactic Protein-3 (MCP-3).

Purification: Affinity chromatography.

Immunogen: Highly pure (>98%) recombinant mMCP-3.

Application Details

Application Notes: ELISA: To detect mMCP-3 by direct ELISA (using 100 l/well antibody solution) this antibody canbe used at a concentration of 0.15-0.30 μg/mL. Used in conjunction with compatiblesecondary reagents, allows the detection of at least 0.2 ng/well of recombinant mMCP-3. Western Blot: To detect mMCP-3 by Western Blot analysis this antibody can be used at a concentration of0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limitfor recombinant mMCP-3 is 1.5-3.0 ng/lane, under either reducing or non-reducingconditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Target Details for CCL7

Target: CCL7 (Chemokine (C-C Motif) Ligand 7 (CCL7))

Alternative Name: MCP3 / CCL7

Background: This is a secreted chemokine which attracts macrophages during inflammation and metastasis. It is a member of the C-C subfamily of chemokines which are characterized by having two adjacent cysteine residues. The protein is an in vivo substrate of matrix metalloproteinase 2, an enzyme which degrades components of the extracellular matrix.Synonyms: C-C motif chemokine 7, CCL-7, MCP-3, Macrophage Chemotactic Protein-3, Monocyte chemoattractant protein 3, Monocyte chemotactic protein 3, NC28, SCYA6, SCYA7, Small-inducible cytokine A7

Gene ID: 20306

NCBI Accession: NP_038682

UniProt: Q03366