KIT Ligand antibody (Biotin)

Catalog No. ABIN115907

The Rabbit Polyclonal anti-KIT Ligand antibody has been validated for WB and EIA. It is suitable to detect KIT Ligand in samples from Mouse.

Quick Overview for KIT Ligand antibody (Biotin) (ABIN115907)

Target: KIT Ligand (KITLG)

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KIT Ligand antibody is conjugated to Biotin

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-KIT Ligand Antibody (Biotin)

Specificity: This antibody reacts with Murine Stem Cell Factor (SCF).

Purification: Affinity chromatography.

Immunogen: Highly pure (>98%) recombinant mSCF.

Application Details

Application Notes: ELISA: To detect mSCF by direct ELISA (using 100 l/well antibody solution) this antibody can beused at a concentration of 0.15-0.30 μg/mL. Used in conjunction with compatiblesecondary reagents, allows the detection of at least 0.2 ng/well of recombinant mSCF. Western Blot: To detect mSCF by Western Blot analysis this antibody can be used at a concentration of0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limitfor recombinant mSCF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Target Details for KIT Ligand

Target: KIT Ligand (KITLG)

Alternative Name: KITLG / SCF

Background: Synonyms: KITL, Kit ligand, MGF, Mast cell growth factor, Stem cell factor, c-Kit ligand

Gene ID: 17311

NCBI Accession: NP_038626

UniProt: P20826

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway