KIT Ligand antibody

Catalog No. ABIN115909

This Rabbit Polyclonal antibody specifically detects KIT Ligand in WB, EIA and Func. It exhibits reactivity toward Mouse.

Quick Overview for KIT Ligand antibody (ABIN115909)

Target: KIT Ligand (KITLG)

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KIT Ligand antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA), Functional Studies (Func)

Product Details anti-KIT Ligand Antibody

Specificity: This antibody recognises Murine Stem Cell Factor.

Purification: Affinity chromatography.

Immunogen: Highly pure (>98%) recombinant mSCF.

Application Details

Application Notes: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of mSCF (25.0 ng/mL),a concentration of 0.60-0.65 μg/mL of this antibody is required. ELISA: To detect mSCF by direct ELISA (using 100 l/well antibody solution) a concentration of atleast 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, inconjunction with compatible secondary reagents, allows the detection of 0.2-0.4 ng/wellof recombinant mSCF. Western Blot: To detect mSCF by Western Blot analysis this antibody can be used at a concentration of0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limitfor recombinant mSCF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Target Details for KIT Ligand

Target: KIT Ligand (KITLG)

Alternative Name: KITLG / SCF

Background: Synonyms: KITL, Kit ligand, MGF, Mast cell growth factor, Stem cell factor, c-Kit ligand

Gene ID: 17311

NCBI Accession: NP_038626

UniProt: P20826

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway