G-CSF antibody (Biotin)

Catalog No. ABIN115923

The Biotin-conjugated Rabbit Polyclonal anti-G-CSF antibody (ABIN115923) specifically detects G-CSF in WB and EIA. The antibody is reactive with Mouse samples.

Quick Overview for G-CSF antibody (Biotin) (ABIN115923)

Target: G-CSF (CSF3) (Colony Stimulating Factor 3 (Granulocyte) (CSF3))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This G-CSF antibody is conjugated to Biotin

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-G-CSF Antibody (Biotin)

Specificity: This antibody reacts with Murine G-CSF.

Purification: Affinity Chromatography

Immunogen: Highly pure (>98%) E.coli derived recombinant Murine G-CSF.

Application Details

Application Notes: ELISA: To detect mG-CSF by direct ELISA (using 100 l/well antibody solution) this antibody canbe used at a concentration of 0.15-0.30 μg/mL. Used in conjunction with compatiblesecondary reagents, allows the detection of at least 0.2 ng/well of recombinant mG-CSF. Western blot: To detect mG-CSF by Western Blot analysis this antibody can be used at a concentration of0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limitfor recombinant mG-CSF is 1.5-3.0 ng/lane, under either reducing or non-reducingconditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody at -20 °C.

Target Details for G-CSF

Target: G-CSF (CSF3) (Colony Stimulating Factor 3 (Granulocyte) (CSF3))

Alternative Name: G-CSF

Background: Granulocyte Colony Stimulating Factor (G-CSF) a cytokine that controls the production, differentiation, and function of granulocytes. It is a potent stimulator of bone marrow cells especially those of neutrophil lineage. In addition, G-CSF can enhance the survival and activate the immunological functions of mature neutrophils.Synonyms: C17orf33, CSF3, Colony stimulating factor 3, GCSF, Granulocyte colony-stimulating factor, Pluripoietin

Gene ID: 12985

NCBI Accession: NP_034101

UniProt: P09920

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization