CCL3 antibody (Biotin)

Catalog No. ABIN115967

The Rabbit Polyclonal anti-CCL3 antibody has been validated for WB and EIA. It is suitable to detect CCL3 in samples from Rat.

Quick Overview for CCL3 antibody (Biotin) (ABIN115967)

Target: CCL3 (Chemokine (C-C Motif) Ligand 3 (CCL3))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CCL3 antibody is conjugated to Biotin

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-CCL3 Antibody (Biotin)

Specificity: Recognises Rat MIP-1 alpha.

Purification: Affinity Chromatography employing an immobilized Rat MIP-1-alpha matrix

Immunogen: Highly pure (>98%) recombinant Rat MIP-1-alpha (Rat MIP-1-alpha).

Application Details

Application Notes: ELISA: To detect rat MIP-1-alpha by direct ELISA (using 100 l/well antibody solution) thisantibody can be used at a concentration of 0.15-0.30 μg/mL. Used in conjunction withcompatible secondary reagents, allows the detection of at least 0.2 ng/well of recombinantrat MIP-1-alpha. Western Blot: To detect rat MIP-1-alpha by Western Blot analysis this antibody can be used at aconcentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant rat MIP-1-alpha is 1.5 - 3.0 ng/lane, under eitherreducing or non-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing. Centrifuge vial prior to opening!

Storage: 4 °C/-20 °C

Storage Comment: Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody at -20 °C.

Target Details for CCL3

Target: CCL3 (Chemokine (C-C Motif) Ligand 3 (CCL3))

Alternative Name: MIP1 alpha / CCL3

Background: Macrophage Inflammatory Protein 1 alpha (MIP1 alpha) and MIP1 beta, two closely related but distinct proteins, were originally co-purified from medium conditioned by a LPS-stimulated murine macrophage cell line. Mature mouse MIP1 alpha shares approximately 77 % and 70 % amino acid identity with human MIP1 alpha and mouse MIP1 beta, respectively. MIP1 proteins are expressed primarily in T cells, B cells, and monocytes after antigen or mitogen stimulation. Has adhesive effects on lymphocytes. MIP1 alpha can inhibit the proliferation of hematopoietic stem cells in vitro as well as in vivo. A signal transducing receptor, designated the C-C chemokine receptor-1 (C-C CKR-1) with seven transmembrane domains that bind MIP1 alpha, MIP1 beta, MCP1 and RANTES with varying affinities, has been isolated.Synonyms: C-C motif chemokine 3, CCL-3, G0/G1 switch regulatory protein 19-1, G0S19-1 protein, LD78 alpha, MIP-1-alpha, MIP1A, Macrophage Inflammatory Protein 1 alpha, SCYA3, Small-inducible cytokine A3, Tonsillar lymphocyte LD78 alpha protein

Gene ID: 25542

NCBI Accession: NP_037157

UniProt: P50229

Pathways: Cellular Response to Molecule of Bacterial Origin, Autophagy