Cardiotrophin 1 antibody (Biotin)

Catalog No. ABIN115987

This Rabbit Polyclonal antibody specifically detects Cardiotrophin 1 in WB and EIA. It exhibits reactivity toward Human.

Quick Overview for Cardiotrophin 1 antibody (Biotin) (ABIN115987)

Target: Cardiotrophin 1 (CTF1)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cardiotrophin 1 antibody is conjugated to Biotin

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-Cardiotrophin 1 Antibody (Biotin)

Specificity: This antibody reacts with Human Cardiotrophin-1.

Purification: Affinity chromatography.

Immunogen: Highly pure (>98%) recombinant hCardiotrophin-1.

Application Details

Application Notes: ELISA: To detect hCardiotrophin-1 by direct ELISA (using 100 l/well antibody solution) thisantibody can be used at a concentration of 0.15-0.30 μg/mL. Used in conjunction withcompatible secondary reagents, allows the detection of at least 0.2 ng/well of recombinanthCardiotrophin-1. Western Blot: To detect hCardiotrophin-1 by Western Blot analysis this antibody can beused at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondaryreagents the detection limit for recombinant hCardiotrophin-1 is 1.5-3.0 ng/lane, undereither reducing or non-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing. Centrifuge vial prior to opening!

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Target Details for Cardiotrophin 1

Target: Cardiotrophin 1 (CTF1)

Alternative Name: Cardiotrophin-1

Background: Cardiotrophin-1 (CT-1), a cardiac hypertrophic factor, is a 21.5 kDa protein member of the IL-6 cytokine family. CT-1 is associated with the pathophysiology of heart diseases, including hypertension, myocardial infarction, valvular heart disease, and congestive heart failure. The protein exerts its cellular effects by interacting with the glycoprotein 130 (gp130)/leukemia inhibitory factor receptor beta (LIFR) heterodimer. In addition, CT-1 activates phosphatidylinositol 3-kinase (PI-3 kinase) in cardiac myocytes and enhances transcription factor NF kappa B DNA -binding activities. CT-1 is highly expressed in the heart, skeletal muscle, prostate and ovary and to lower levels in lung, kidney, pancreas, thymus, testis and small intestine.Synonyms: CT-1, CTF1

Gene ID: 1489

NCBI Accession: NP_001136016

UniProt: Q16619

Pathways: JAK-STAT Signaling