EGF antibody

Catalog No. ABIN115993

This Rabbit Polyclonal antibody specifically detects EGF in WB, IHC (p), EIA, IHC (fro) and Func. It exhibits reactivity toward Human.

Quick Overview for EGF antibody (ABIN115993)

Target: EGF (Epidermal Growth Factor (EGF))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EGF antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Functional Studies (Func)

Product Details anti-EGF Antibody

Specificity: Human Epidermal Growth Factor (hEGF)

Purification: Affinity Chromatography using an immobilized Human EGF matrix

Immunogen: Highly pure (>98%) E.coli derived recombinant Human EGF (hEGF).

Application Details

Application Notes: ELISA: To detect hEGF by direct ELISA (using 100 l/well antibody solution) a concentrationof at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, inconjunction with compatible secondary reagents, allows the detection of 0.2-0.4 ng/wellof recombinant hEGF. Western Blot: To detect hEGF by Western Blot analysis this antibody can be used at aconcentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hEGF is 1.5-3.0 ng/lane, under either reducing ornon-reducing conditions. Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofhEGF (2.0 ng/mL), a concentration of 0.25-0.35 μg/mL of this antibody is required. Immunohistochemistry: A starting dilution of 1 μg/mL is recommended for frozen sections. For paraffin embedded sections use microwave pretreatment, EDTA, pH 9 and a dilution of1-5 μg/mL.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.2 without preservatives.

Preservative: Without preservative

Handling Advice: Centrifuge vial prior to opening!

Storage: 4 °C

Target Details for EGF

Target: EGF (Epidermal Growth Factor (EGF))

Alternative Name: EGF

Background: Epidermal growth factor (EGF) has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells. The EGF precursor is believed to exist as a membrane bound molecule which is proteolytically cleaved to generate the 53 amino acid peptide hormone that stimulates cells to divide. EGF exerts its actions by binding to the EGFR, a 170 kDa protein. Epidermal growth factor (EGF) is a key growth factor regulating cell survival. Through its binding to cell surface receptors, EGF activates an extensive network of signal transduction pathways that include activation of the PI3K/AKT, RAS/ERK and JAK/STAT pathways. Because of its key role in driving the proliferation of cells, EGFR is a target of several anti-cancer drugs currently in development.Synonyms: Epidermal growth factor, HOMG4, Pro-epidermal growth factor, URG, Urogastrone

Gene ID: 1950

NCBI Accession: NP_001954

UniProt: P01133

Pathways: NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation