FGF1 antibody (Biotin)

Catalog No. ABIN116003

The Rabbit Polyclonal anti-FGF1 antibody has been validated for WB and EIA. It is suitable to detect FGF1 in samples from Human.

Quick Overview for FGF1 antibody (Biotin) (ABIN116003)

Target: FGF1 (Fibroblast Growth Factor 1 (Acidic) (FGF1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FGF1 antibody is conjugated to Biotin

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-FGF1 Antibody (Biotin)

Specificity: Human FGF-acidic.

Purification: Affinity Chromatography.

Immunogen: Highly pure (>98%) E.Coli derived recombinant Human FGF-acidic.

Application Details

Application Notes: ELISA: To detect hFGF-acidic by direct ELISA (using 100 l/well antibody solution) thisantibody can be used at a concentration of 0.15 - 0.30 μg/mL. Used in conjunction withcompatible secondary reagents, allows the detection of at least 0.2 ng/well of recombinanthFGF-acidic. Western Blot: To detect hFGF-acidic by Western Blot analysis this antibody can be used at aconcentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hFGF-acidic is 1.5 - 3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Preservative: Without preservative

Handling Advice: Centrifuge vial prior to opening!

Storage: 4 °C

Target Details for FGF1

Target: FGF1 (Fibroblast Growth Factor 1 (Acidic) (FGF1))

Alternative Name: FGF Acidic / FGF1

Background: The heparin-binding growth factors are angiogenic agents in vivo and are potent mitogens for a variety of cell types in vitro. There are differences in the tissue distribution and concentration of these two growth factors.Synonyms: Acidic fibroblast growth factor, Beta-endothelial cell growth factor, ECGF-beta, FGFA, HBGF-1, HBGF1, Heparin-binding growth factor 1

Gene ID: 2246

NCBI Accession: NP_000791

UniProt: P05230

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway