G-CSF antibody

Catalog No. ABIN116025

The Rabbit Polyclonal anti-G-CSF antibody (ABIN116025) specifically detects G-CSF in WB, IHC (p), EIA and Func. The antibody is reactive with Human samples.

Quick Overview for G-CSF antibody (ABIN116025)

Target: G-CSF (CSF3) (Colony Stimulating Factor 3 (Granulocyte) (CSF3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This G-CSF antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA), Functional Studies (Func)

Product Details anti-G-CSF Antibody

Specificity: Recognizes Granulocyte Colony Stimulating Factor (G-CSF).

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Affinity Chromatography employing immobilized hG-CSF matrix.

Immunogen: Highly purified E.Coli derived recombinant Human G-CSF (Cat.-No PA060)

Application Details

Application Notes: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofHuman G-CSF (0.5 ng/mL), a concentration of 0.04-0.06 μg/mL of this antibody is required. Sandwich ELISA: To detect Human G-CSF by Sandwich ELISA (using 100 μL/well antibodysolution) a concentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinitypurified antibody, in conjunction with Biotinylated Anti-Human G-CSF (PP1011B) as adetection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant HumanG-CSF. Western Blot: To detect Human G-CSF by Western Blot analysis this antibody can be usedat a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondaryreagents the detection limit for recombinant hG-CSF is 1.5-3.0 ng/lane, under eitherreducing or non-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for G-CSF

Target: G-CSF (CSF3) (Colony Stimulating Factor 3 (Granulocyte) (CSF3))

Alternative Name: G-CSF

Background: Granulocyte Colony Stimulating Factor (G-CSF) a cytokine that controls the production, differentiation, and function of granulocytes. It is a potent stimulator of bone marrow cells especially those of neutrophil lineage. In addition, G-CSF can enhance the survival and activate the immunological functions of mature neutrophils.Synonyms: C17orf33, CSF3, Colony stimulating factor 3, GCSF, Granulocyte colony-stimulating factor, Pluripoietin

Gene ID: 1440

NCBI Accession: NP_000750

UniProt: P09919

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization