CXCL2 antibody

Catalog No. ABIN116031

Product Details anti-CXCL2 Antibody

Target: CXCL2 (Chemokine (C-X-C Motif) Ligand 2 (CXCL2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CXCL2 antibody is un-conjugated

Application: Western Blotting (WB), Functional Studies (Func), Enzyme Immunoassay (EIA)

Specificity: This antibody detects GRO-beta.

Purification: Affinity Chromatography employing immobilized hGRO-beta matrix.

Immunogen: Highly pure (>98%) recombinant hGRO-beta (human GRO-beta)

Application Details

Application Notes: Neutralization(To yield one-half maximal inhibition [ND50] of the biological activity of hGRO-beta (100.00 ng/mL), a concentration of 0.5-1.0 μg/mL of this antibody has been used with previous lots)ELISA(Indirect): To detect hGRO-beta by indirect ELISA (using 100 μL/well antibody solution) aconcentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with compatible secondary reagents, allows the detection of atleast 0.2-0.4 ng/well of recombinant hGRO-beta. (Sandwich): To detect hGRO-beta by sandwich ELISA (using 100 μL/well antibody solution)a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with biotinylated anti-Human GRO-beta as a detection antibody,allows the detection of at least 0.2 - 0.4 ng/well of recombinant hGRO-beta. Western BlotTo detect hGRO-beta by Western Blot analysis this antibody can be used at a concentrationof 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detectionlimit for recombinant hGRO-beta is 1.5-3.0 ng/lane, under either reducing or non-reducing

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: The lyophilized antibody is stable at -20 °C for one year from despatch. The reconstituted antibody is stable for two weeks at 2-8 °C. Frozen aliquots are stable for six months when stored at -20 °C.

Expiry Date: 12 months

Target Details for CXCL2

Target: CXCL2 (Chemokine (C-X-C Motif) Ligand 2 (CXCL2))

Alternative Name: CXCL2 / MIP2A (CXCL2 Products)

Synonyms: CINC-2a antibody, GRO2 antibody, GROb antibody, MGSA-b antibody, MIP-2a antibody, MIP2 antibody, MIP2A antibody, SCYB2 antibody, GRO3 antibody, Gro2 antibody, MIP-2 antibody, Mgsa-b antibody, Mip2 antibody, Scyb antibody, Scyb2 antibody, Mip-2 antibody, GROB antibody, MIP-2alpha antibody, C-X-C motif chemokine ligand 2 antibody, chemokine (C-X-C motif) ligand 2 antibody, CXCL2 antibody, Cxcl2 antibody

Background: GRO beta is a member of the CXC, or chemokine class. It contains the ELR domain immediately preceding the first cysteine residue near the amino terminus. Other chemokines in this group include IL8, GRO alpha/beta/gamma, mouse KC, ENA78, GCP2, PBP/CTAPIII/beta TG/NAP2. These chemokines act primarily on neutrophils as chemoattractants and activators, including neutrophil degradation with release of myloperoxidase and other enzymes. GRO beta was originally identified as a heparin-binding protein secreted from a murine macrophage cell line in response to endotoxin stimulation. GRO beta is an approximately 8 kDa polypeptide of 73 amino acids. The precursor form of GRO beta consists of 100 amino acids. To generate the mature GRO beta, the precursor cleaves its amino terminal 27 amino acids. GRO beta shows 60 % amino acid homology to human GRO alpha and GRO gamma.Synonyms: C-X-C motif chemokine 2, GRO2, GROB, Gro-beta, Growth-regulated protein beta, MIP-2A, MIP2-alpha, Macrophage inflammatory protein 2-alpha, SCYB2

Gene ID: 2920

NCBI Accession: NP_002080

UniProt: P19875

Pathways: Cellular Response to Molecule of Bacterial Origin