CXCL3 antibody

Catalog No. ABIN116036

The Rabbit Polyclonal anti-CXCL3 antibody is suitable to detect CXCL3 in samples from Human. It has been validated for WB, EIA, Func and IHC (p).

Quick Overview for CXCL3 antibody (ABIN116036)

Target: CXCL3 (Chemokine (C-X-C Motif) Ligand 3 (CXCL3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CXCL3 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA), Functional Studies (Func), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-CXCL3 Antibody

Specificity: Human Growth Related Oncoprotein g (hGRO gamma)

Purification: Affinity Chromatography.

Immunogen: Highly pure (>98%) recombinant hGRO-gamma (human GRO-gamma)

Application Details

Application Notes: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hGRO-gamma (100 ng/mL), a concentration of 1.0-2.0 μg/mL of this antibody is required. ELISAIndirect: To detect hGRO-gamma by indirect ELISA (using 100 μL/well antibody solution) aconcentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with compatible secondary reagents, allows the detection of atleast 0.2-0.4 ng/well of recombinant hGRO-gamma. Sandwich: To detect hGRO-gamma by sandwich ELISA (using 100 μL/well antibodysolution) a concentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinitypurified antibody, in conjunction with Biotinylated Anti-Human GRO-gamma (PP1014B1 orPP1014B2) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well ofrecombinant hGRO-gamma. Western BlotTo detect hGRO-gamma by Western Blot analysis this antibody can be used at aconcentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hGRO-gamma is 1.5-3.0 ng/lane, under either reducingor non-reducing conditions. Immunohistochemistry on Formalin-Fixed Paraffin-Embedded Sections: 0.7-0.5 μg/mL withan overnight incubation at 4 °C. An HRP-labeled polymer detection system was used with aDAB chromogen. Heat induced antigen retrieval with a pH 6.0 Sodium Citrate buffer is

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives

Preservative: Without preservative

Handling Advice: This product is photosensitive and should be protected from light

Storage: 4 °C

Storage Comment: Store the antibody at 2-8 °C. DO NOT FREEZE.

Target Details for CXCL3

Target: CXCL3 (Chemokine (C-X-C Motif) Ligand 3 (CXCL3))

Alternative Name: CXCL3 / MIP2B

Background: Growth Regulated Protein gamma (GRO gamma) is a cytokine that may play a role in inflammation and affects endothelial cells in an autocrine fashion. It belongs to the intercrine alpha family (small cytokine c-x-c) (chemokine cxc).Synonyms: C-X-C motif chemokine 3, GRO-gamma, GRO3, GROG, Growth-regulated protein gamma, MIP2-beta, Macrophage inflammatory protein 2-beta, SCYB3

Gene ID: 2921

NCBI Accession: NP_002081

UniProt: P19876

Pathways: Cellular Response to Molecule of Bacterial Origin, Autophagy