IGF2 antibody

Catalog No. ABIN116059

This Rabbit Polyclonal antibody specifically detects IGF2 in WB, IHC (p), Func and EIA. It exhibits reactivity toward Human.

Quick Overview for IGF2 antibody (ABIN116059)

Target: IGF2 (Insulin-Like Growth Factor 2 (IGF2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IGF2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Functional Studies (Func), Enzyme Immunoassay (EIA)

Product Details anti-IGF2 Antibody

Purification: Affinity chromatography employing immobilized hhIGF-II matrix

Immunogen: Highly pure (>98%) recombinant human IGF-II.

Application Details

Application Notes: NeutralizationTo yield one-half maximal inhibition [ND50] of the biological activity of hIGF-II (10 ng/mL), aconcentration of 3.1 - 4.6 μg/mL of this antibody is required. ElisaIndirect: To detect hIGF-II by indirect ELISA (using 100 μL/well antibody solution) aconcentration of 0.5 - 2.0 μg/mL is required. In conjunction with compatible secondaryreagents, it allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIGF-II. Sandwich: To detect hIGF-II by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.5 - 2.0 μg/mL is required. In conjunction with Biotinylated Anti-HumanIGF-II as a detection antibody, it allows the detection of at least 0.2 - 0.4 ng/well ofrecombinant hIGF-II. Western BlotTo detect hIGF-II by Western Blot analysis this antibody can be used at a concentration of0.1- 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limitfor recombinant hIGF-II is 1.5-3.0 ng/lane, under either reducing or non-reducingconditions.

Restrictions: For Research Use only

Handling

Reconstitution: Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2

Handling Advice: This product is photosensitive and should be protected from light. Should it contain a precipitate we recommend microcentrifugation before use.

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C. DO NOT FREEZE!

Target Details for IGF2

Target: IGF2 (Insulin-Like Growth Factor 2 (IGF2))

Alternative Name: Insulin-Like Growth Factor II / IGF2

Background: The IGF2 gene encodes a member of the insulin family of polypeptide growth factors that is involved in development and growth. It is expressed only from the paternally inherited allele and is an imprinted gene which is a candidate gene for eating disorders. There is a read-through, INS-IGF2, which aligns to this gene at the 3' region and to the upstream INS gene at the 5' region. Two alternatively spliced transcript variants encoding the same protein have been found for this gene. Insulin Like Growth Factor-2 (IGF-2) is a polypeptide growth factor, which stimulates the proliferation of a wide range of cell types.Synonyms: IGF-II, Somatomedin-A

Gene ID: 3481

NCBI Accession: NP_000603

UniProt: P01344

Pathways: Hormone Activity, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Regulation of Carbohydrate Metabolic Process, Activated T Cell Proliferation