IL1A antibody (Biotin)

Catalog No. ABIN116061

This anti-IL1A antibody is a Rabbit Polyclonal antibody detecting IL1A in WB and EIA. Suitable for Human.

Quick Overview for IL1A antibody (Biotin) (ABIN116061)

Target: IL1A (Interleukin 1 alpha (IL1A))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL1A antibody is conjugated to Biotin

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-IL1A Antibody (Biotin)

Specificity: This antibody recognizes Human Interleukin-1 alpha (IL-1 alpha).

Purification: Affinity Chromatography.

Immunogen: Highly pure (>98%) recombinant hIL-1 alpha (human IL-1 alpha).

Application Details

Application Notes: ELISA: To detect hIL-1 alpha by direct ELISA (using 100 l/well antibody solution) thisantibody can be used at a concentration of 0.15 - 0.30 μg/mL. Used in conjunction withcompatible secondary reagents, allows the detection of at least 0.2 ng/well of recombinanthIL-1 alpha. Western Blot: To detect hIL-1 alpha by Western Blot analysis this antibody can be used at aconcentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hIL-1 alpha is 1.5 - 3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Preservative: Without preservative

Handling Advice: Centrifuge vial prior to opening! This product is photosensitive and should be protected from light

Storage: 4 °C

Storage Comment: Prior to and following reconstitution store the antibody undiluted at 2-8 °C. DO NOT FREEZE!

Target Details for IL1A

Target: IL1A (Interleukin 1 alpha (IL1A))

Alternative Name: Interleukin-1 alpha / IL-1A

Background: Interleukins (ILs) are a large group of cytokines that are produced mainly by leukocytes, although some are produced by certain phagocytes and auxiliary cells. Each IL acts on a specific, limited group of cells through a receptor specific for that IL. Interleukin 1 (IL1), originally known as lymphocyte activating factor (LAF), activates T cells and lymphocytes, which then proliferate and secrete interleukin 2. IL1 is primarily released from stimulated macrophages and monocytes, but also is released from several other cell types and is thought to play a key role in inflammatory and immune responses. The two closely related agents, interleukin 1 alpha (IL1 alpha) and interleukin 1 beta (IL1 beta) bind to the same cell surface receptor, elicit nearly identical biological responses and share 25 % homology in their amino acid sequence.Synonyms: Hematopoietin-1, IL-1 alpha, IL1A, IL1F1

Gene ID: 3552

NCBI Accession: NP_000566

UniProt: P01583

Pathways: NF-kappaB Signaling, Autophagy, Cancer Immune Checkpoints