IL-2 antibody

Catalog No. ABIN116067

This anti-IL-2 antibody is a Rabbit Polyclonal antibody detecting IL-2 in WB, EIA, Func and IHC (p). Suitable for Human.

Quick Overview for IL-2 antibody (ABIN116067)

Target: IL-2 (IL2) (Interleukin 2 (IL2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL-2 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA), Functional Studies (Func), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-IL-2 Antibody

Purification: Affinity Chromatography.

Immunogen: Highly purified (>98%) recombinant human Interleukin 2. Remarks: Mol. Weight: 150 kDa

Application Details

Application Notes: NeutralisationTo yield one-half maximal inhibition [ND50] of the biological activity of Human IL-2 (2.0 ng/mL), a concentration of 0.12-0.16 μg/mL of this antibody is required. ELISAIndirect: To detect hIL-2 by indirect ELISA (using 100 μL/well antibody solution) a concentration of0.5-2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, inconjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant Human IL-2. Sandwich: To detect hIL-2 by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with Biotinylated Anti-Human IL-2 (PP1024B1 or PP1024B2) as adetection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hIL-2. Western blotTo detect hIL-2 by Western Blot analysis this antibody can be used at a concentration of0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limitfor recombinant Human IL-2 is 1.5-3.0 ng/lane, under either reducing or non-reducingconditions.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing. Centrifuge vial prior to opening!

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Target Details for IL-2

Target: IL-2 (IL2) (Interleukin 2 (IL2))

Alternative Name: Interleukin-2 / IL2

Background: Human interleukin 2 (IL2) is a secreted cytokine that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine is a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL4) and interleukin 7 (IL7). The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli. IL2 has been shown to have antitumor effects in some studies. This is probably mediated by cytotoxic effector cells.Synonyms: IL-2, T-cell growth factor, TCGF

Gene ID: 9606

UniProt: P60568

Pathways: JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation