IL-9 antibody (Biotin)

Catalog No. ABIN116093

The Rabbit Polyclonal anti-IL-9 antibody has been validated for WB and EIA. It is suitable to detect IL-9 in samples from Human.

Quick Overview for IL-9 antibody (Biotin) (ABIN116093)

Target: IL-9 (IL9) (Interleukin 9 (IL9))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL-9 antibody is conjugated to Biotin

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-IL-9 Antibody (Biotin)

Specificity: Human Interleukin-9.

Purification: Affinity chromatography.

Immunogen: Highly pure (>98%) recombinant hIL-9 (human IL-9).

Application Details

Application Notes: ELISA: To detect hIL-9 by direct ELISA (using 100 l/well antibody solution) this antibodycan be used at a concentration of 0.15 - 0.30 μg/mL. Used in conjunction with compatiblesecondary reagents, allows the detection of at least 0.2 ng/well of recombinant hIL-9. Western Blot: To detect hIL-9 by Western Blot analysis this antibody can be used at aconcentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hIL-9 is 1.5 - 3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing. Centrifuge vial prior to opening!

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 4-8 °C for one month or at -20 °C for longer. This product should be stored undiluted.

Target Details for IL-9

Target: IL-9 (IL9) (Interleukin 9 (IL9))

Alternative Name: Interleukin-9 / IL9

Background: Interleukin 9 (IL9) is a cytokine preferentially produced by T helper type 2 lymphocytes and active on various cell types such as T and B lymphocytes, mast cells and haemopoietic progenitors, but no effect of this cytokine has been reported so far on mononuclear phagocytes. IL9 stimulates cell proliferation and prevents apoptosis. The IL9 receptor (IL 9R) belongs to the haemopoietic receptor superfamily and its signal transduction involves mainly the (JAK/STAT) pathway. Some studies have implicated IL9 as an important cytokine in a number of Th2 cytokine mediated pathologies, in particular asthma.Synonyms: IL-9, P40 cytokine, T-cell growth factor P40

Gene ID: 3578

NCBI Accession: NP_000581

UniProt: P15248

Pathways: JAK-STAT Signaling