IL16 antibody

Catalog No. ABIN116115

The Rabbit Polyclonal anti-IL16 antibody (ABIN116115) specifically detects IL16 in WB, EIA and Func. The antibody is reactive with Human samples.

Quick Overview for IL16 antibody (ABIN116115)

Target: IL16 (Interleukin 16 (IL16))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL16 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA), Functional Studies (Func)

Product Details anti-IL16 Antibody

Specificity: Reacts with Human Interleukin-16. Other species not tested.

Purification: Affinity chromatography.

Immunogen: Highly pure (>98%) recombinant hIL-16 (human Interleukin-16).

Application Details

Application Notes: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofhIL-16 (4.20 ng/mL), a concentration of 0.07-0.012 μg/mL of this antibody is required. ELISA: To detect hIL-16 by direct ELISA (using 100l/well antibody solution) a concentrationof at least 0.5g/mL of this antibody is required. This antigen affinity purified antibody, inconjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/wellof recombinant hIL-16. Western Blot: To detect hIL-16 by Western Blot analysis this antibody can be used at aconcentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hIL-16 is 1.5-3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing. Centrifuge vial prior to opening!

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Target Details for IL16

Target: IL16 (Interleukin 16 (IL16))

Alternative Name: Interleukin-16 / IL16

Background: IL16 is a proinflammatory cytokine that is chemotactic for CD4+ T lymphocytes, monocytes, and eosinophils. IL16 is synthesized as a precursor molecule (pro IL16) of approximately 68 kDa and 631 amino acid residues lacking a signal peptide. The sequence and structure of IL16 is conserved across species. In addition to inducing chemotaxis, IL16 upregulates the IL2 receptor and upregulates HLADR expression. It also inhibits T cell receptor (TCR)/CD3 dependent activation, and suppresses HIV1 replication in vitro. Sources of IL16 include epithelial cells, mast cells, T lymphocytes (CD4+ and CD8+), macrophages, synovial fibroblasts, and eosinophils.Synonyms: IL-16, LCF, Lymphocyte chemoattractant factor

Gene ID: 3603

NCBI Accession: NP_001165599

UniProt: Q14005