CCL7 antibody (Biotin)

Catalog No. ABIN116145

This anti-CCL7 antibody is a Goat Polyclonal antibody detecting CCL7 in WB and EIA. Suitable for Human.

Quick Overview for CCL7 antibody (Biotin) (ABIN116145)

Target: CCL7 (Chemokine (C-C Motif) Ligand 7 (CCL7))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This CCL7 antibody is conjugated to Biotin

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-CCL7 Antibody (Biotin)

Specificity: This antibody recognizes Human Macrophage Chemotactic Protein-3 (MCP-3/CCL7). Other species not tested.

Purification: Affinity chromatography.

Immunogen: Highly pure (>98%) recombinant hMCP-3 (human MCP-3).

Application Details

Application Notes: ELISA: To detect hMCP-3 by direct ELISA (using 100 l/well antibody solution) this antibodycan be used at a concentration of 0.15 - 0.30 μg/mL. Used in conjunction with compatiblesecondary reagents, allows the detection of at least 0.2 ng/well of recombinant hMCP-3. Western Blot: To detect hMCP-3 by Western Blot analysis this antibody can be used at aconcentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hMCP-3 is 1.5 - 3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. Add. Information: Centrifuge vial prior to opening!

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Target Details for CCL7

Target: CCL7 (Chemokine (C-C Motif) Ligand 7 (CCL7))

Alternative Name: MCP3 / CCL7

Background: This is a secreted chemokine which attracts macrophages during inflammation and metastasis. It is a member of the C-C subfamily of chemokines which are characterized by having two adjacent cysteine residues. The protein is an in vivo substrate of matrix metalloproteinase 2, an enzyme which degrades components of the extracellular matrix.Synonyms: C-C motif chemokine 7, CCL-7, MCP-3, Macrophage Chemotactic Protein-3, Monocyte chemoattractant protein 3, Monocyte chemotactic protein 3, NC28, SCYA6, SCYA7, Small-inducible cytokine A7

Gene ID: 6354

NCBI Accession: NP_006264

UniProt: P80098