CCL7 antibody

Catalog No. ABIN116147

The Goat Polyclonal anti-CCL7 antibody has been validated for WB and EIA. It is suitable to detect CCL7 in samples from Human.

Quick Overview for CCL7 antibody (ABIN116147)

Target: CCL7 (Chemokine (C-C Motif) Ligand 7 (CCL7))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This CCL7 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-CCL7 Antibody

Specificity: This antibody recognizes Human Macrophage Chemotactic Protein-3 (MCP-3/CCL7). Other species not tested.

Purification: Affinity chromatography.

Immunogen: Highly pure (>98%) recombinant hMCP?3 (human Macrophage/Monocyte chemotactic protein-3).

Application Details

Application Notes: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofhMCP-3 (100.0 ng/mL), a concentration of 3.0-4.0 μg/mL of this antibody is required. ELISA: (Indirect): To detect hMCP-3 by indirect ELISA (using 100 μL/well antibody solution) aconcentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with compatible secondary reagents, allows the detection of atleast 0.2-0.4 ng/well of recombinant hMCP-3. (Sandwich): To detect hMCP-3 by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with Biotinylated Anti-Human MCP-3 (PP1046B) as a detectionantibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hMCP-3. Western Blot: To detect hMCP-3 by Western Blot analysis this antibody can be used at aconcentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hMCP-3 is 1.5-3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives

Preservative: Without preservative

Handling Advice: Centrifuge vial prior to opening!

Storage: 4 °C

Storage Comment: Store the antibody in the dark at 2-8 °C. Do Not Freeze! Avoid prolonged exposure to light.

Target Details for CCL7

Target: CCL7 (Chemokine (C-C Motif) Ligand 7 (CCL7))

Alternative Name: MCP3 / CCL7

Background: This is a secreted chemokine which attracts macrophages during inflammation and metastasis. It is a member of the C-C subfamily of chemokines which are characterized by having two adjacent cysteine residues. The protein is an in vivo substrate of matrix metalloproteinase 2, an enzyme which degrades components of the extracellular matrix.Synonyms: C-C motif chemokine 7, CCL-7, MCP-3, Macrophage Chemotactic Protein-3, Monocyte chemoattractant protein 3, Monocyte chemotactic protein 3, NC28, SCYA6, SCYA7, Small-inducible cytokine A7

Gene ID: 6354

NCBI Accession: NP_006264

UniProt: P80098