CCL13 antibody

Catalog No. ABIN116151

The Rabbit Polyclonal anti-CCL13 antibody (ABIN116151) specifically detects CCL13 in WB, EIA and Func. The antibody is reactive with Human samples.

Quick Overview for CCL13 antibody (ABIN116151)

Target: CCL13 (Chemokine (C-C Motif) Ligand 13 (CCL13))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CCL13 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA), Functional Studies (Func)

Product Details anti-CCL13 Antibody

Specificity: Human Macrophage Chemotactic Protein-4 (MCP-4) (NCC-1).

Purification: Affinity chromatography.

Immunogen: Highly pure (>98%) recombinant hMCP-4.

Application Details

Application Notes: Neutralization: To yield one-half maximal inhibition [ND50 ] of the biological activity ofhMCP-4 (30.0 ng/mL), a concentration of 1.0-1.5 μg/mL of this antibody is required. ELISA: To detect hMCP-4 by direct ELISA (using 100 l/well antibody solution) aconcentration of at least 0.5 μg/mL of this antibody is required. This antigen affinitypurified antibody, in conjunction with compatible secondary reagents, allows the detectionof recombinant hMCP-4. Western Blot: To detect hMCP-4 by Western Blot analysis this antibody can be used at aconcentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hMCP-4 is 1.5-3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Preservative: Without preservative

Handling Advice: Centrifuge vial prior to opening!

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody lyophilized at 2-8 °C and reconstituted at 2-8 °C for one week or (in aliquots) at -20 °C for longer. If a slight precipitation occurs upon storage, this should be removed by centrifugation.

Target Details for CCL13

Target: CCL13 (Chemokine (C-C Motif) Ligand 13 (CCL13))

Alternative Name: MCP-4 / CCL13

Background: Human macrophage/monocyte chemotactic protein 4 (MCP4) is a member of the b-chemokine family (C-C) of Cytokines. Like Eotaxin, MCP4 is a powerful eosinophil chemo-attractant. Additionally, like MCP3, MCP4 elicits chemo-attraction of monocytes and T lymphocytes.Synonyms: C-C motif chemokine 13, CCL13, CK-beta-10, MCP4, Monocyte chemotactic protein 4, NCC-1, NCC1, SCYA13, Small-inducible cytokine A13

Gene ID: 6357

NCBI Accession: NP_005399

UniProt: Q99616

Pathways: Regulation of Systemic Arterial Blood Pressure by Hormones, The Global Phosphorylation Landscape of SARS-CoV-2 Infection