M-CSF/CSF1 antibody (Biotin)

Catalog No. ABIN116153

The Rabbit Polyclonal anti-M-CSF/CSF1 antibody has been validated for WB and EIA. It is suitable to detect M-CSF/CSF1 in samples from Human.

Quick Overview for M-CSF/CSF1 antibody (Biotin) (ABIN116153)

Target: M-CSF/CSF1 (CSF1) (Colony Stimulating Factor 1 (Macrophage) (CSF1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This M-CSF/CSF1 antibody is conjugated to Biotin

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-M-CSF/CSF1 Antibody (Biotin)

Specificity: Recognizes Human Macrophage Colony Stimulating Factor (M-CSF).

Purification: Affinity Chromatography

Immunogen: Highly pure (>98%) E.coli derived recombinant Human M-CSF (hM-CSF)

Application Details

Application Notes: ELISA: To detect hM-CSF by direct ELISA (using 100 l/well antibody solution) this antibodycan be used at a concentration of 0.15 - 0.30 μg/mL. Used in conjunction with compatiblesecondary reagents, allows the detection of at least 0.2 ng/well of recombinant hM-CSF. Western Blot: To detect hM-CSF by Western Blot analysis this antibody can be used at aconcentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hM-CSF is 1.5 - 3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing. Centrifuge vial prior to opening!

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Target Details for M-CSF/CSF1

Target: M-CSF/CSF1 (CSF1) (Colony Stimulating Factor 1 (Macrophage) (CSF1))

Alternative Name: Macrophage Colony Stimulating Factor (M-CSF)

Background: Synonyms: CSF-1, CSF1, MCSF, Macrophage colony-stimulating factor 1

Gene ID: 1435

NCBI Accession: NP_000748

UniProt: P09603

Pathways: RTK Signaling