PDGFA antibody

Catalog No. ABIN116207

The Rabbit Polyclonal anti-PDGFA antibody has been validated for WB, IHC (p), EIA and Func. It is suitable to detect PDGFA in samples from Human.

Quick Overview for PDGFA antibody (ABIN116207)

Target: PDGFA (Platelet Derived Growth Factor A (PDGFA))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PDGFA antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA), Functional Studies (Func)

Product Details anti-PDGFA Antibody

Specificity: The antibody recognizes the human Platelet Derived Growth Factor AA (hPDGF-AA). Not tested in other species.

Purification: Immunoaffinity chromatography using immobilized human PDGF matrix

Immunogen: Highly pure (>98%) recombinant hPDGF-AA

Application Details

Application Notes: Neutralisation: To yield one-half maximal inhibition [ND50] of the biological activity of hPDGF-AA (5.0 ng/mL),a concentration of 0.05 - 0.07 μg/mL of this antibody is required. Immunohistochemistry: The antibody stained formalin-fixed, paraffin embedded sections of normal human skin at1.0 μg/mL overnight at 4C. (A HRP-labeled polymer detection system was used with anon-alcohol soluble AEC chromogen and a proteinase K antigen retrieval). ELISA: Indirect - To detect hPDGF-AA by indirect ELISA (using 100 μL/well antibody solution),a concentration of 0.5 - 2.0 μg/mL of this antibody is required, allows the detection of atleast 0.2 - 0.4 ng/well of recombinant hPDGF-AA. Sandwich - To detect hPDGF-AA by sandwich ELISA (using 100 μL/well antibody solution),a concentration of 0.5 - 2.0 μg/mL of this antibody is required, in conjunction with abiotinylated anti-human PDGF-AA (PP1061B) as the detection antibody at least 0.2 - 0.4 ng/well of recombinant hPDGF-AA can be detected. Western blot: To detect hPDGF-AA by Western blot analysis this antibody can be used at a concentrationof 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detectionlimit for recombinant hPDGF-AA is 1.5 -3.0 ng/lane, under either reducing or non-reducingconditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Centrifuge vial prior to opening. Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2, without preservatives

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: The lyophilized antibody is stable at -20 °C for one year from despatch. The reconstituted antibody can be stored for two weeks at 2-8 °C. Frozen aliquots are stable for six months when stored at -20 °C.

Expiry Date: 12 months

Target Details for PDGFA

Target: PDGFA (Platelet Derived Growth Factor A (PDGFA))

Alternative Name: PDGFA

Background: Synonyms: PDGF A, PDGF alpha, PDGF subunit A, PDGF-1, PDGF-A, PDGF1, Platelet-derived growth factor A chain, Platelet-derived growth factor alpha, Platelet-derived growth factor subunit A

Gene ID: 5154

NCBI Accession: NP_002598

UniProt: P04085

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Smooth Muscle Cell Migration, Platelet-derived growth Factor Receptor Signaling