PDGFB antibody (Biotin)

Catalog No. ABIN116210

The Rabbit Polyclonal anti-PDGFB antibody is suitable to detect PDGFB in samples from Human. It has been validated for WB and EIA.

Quick Overview for PDGFB antibody (Biotin) (ABIN116210)

Target: PDGFB (Platelet Derived Growth Factor Subunit B (PDGFB))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PDGFB antibody is conjugated to Biotin

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-PDGFB Antibody (Biotin)

Specificity: Recognizes Human Platelet Derived Growth Factor BB (hPDGF-BB)

Purification: Affinity Chromatography.

Immunogen: Highly pure (>98%) Recombinant Human PDGF-B.

Application Details

Application Notes: Direct ELISA: To detect Human PDGF-BB by direct ELISA (using 100 μL/well antibodysolution) this antibody can be used at a concentration of approx. 1.0 μg/mL. This Biotinylatedantibody allows the detection of at least 0.2 ng/well of recombinant hPDGF-BB. Sandwich ELISA: To detect Human PDGF-BB by Sandwich ELISA (using 100 μL/well antibodysolution) this antibody can be used at a concentration of 0.25-1.0 μg/mL. This Biotinylatedantibody, in conjunction with anti-Human PDGF-BB antibody (PP1062P1 or PP1062P2) as aCapture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinanthPDGF-BB. Western Blot: To detect hPDGF-BB by Western Blot analysis this antibody can be used at aconcentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hPDGF-BB is 1.5-3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Target Details for PDGFB

Target: PDGFB (Platelet Derived Growth Factor Subunit B (PDGFB))

Alternative Name: PDGFB

Background: Platelet Derived Growth Factor (PDGF) is the major serum mitogen for cells of mesenchymal origin in humans. The biologically active protein is a dimer composed of two related polypeptides designated A and B, the dimers being connected by disulfide bonds. The PDGF protein has been implicated both directly as well as indirectly in several pathological states including neoplasia, arthritis, arteriosclerosis and bone marrow sclerosis.Synonyms: Becaplermin, PDGF B, PDGF beta, PDGF subunit B, PDGF-2, PDGF-B, PDGF2, Platelet-derived growth factor B chain, Platelet-derived growth factor beta, Platelet-derived growth factor subunit B, SIS, c-Sis

Gene ID: 5155

NCBI Accession: NP_002599

UniProt: P01127

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Carbohydrate Metabolic Process, Smooth Muscle Cell Migration, Platelet-derived growth Factor Receptor Signaling