KIT Ligand antibody

Catalog No. ABIN116225

The Rabbit Polyclonal anti-KIT Ligand antibody has been validated for WB, EIA, IHC (p) and Func. It is suitable to detect KIT Ligand in samples from Human.

Quick Overview for KIT Ligand antibody (ABIN116225)

Target: KIT Ligand (KITLG)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KIT Ligand antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Functional Studies (Func)

Product Details anti-KIT Ligand Antibody

Specificity: Human Stem Cell Factor (SCF).

Purification: Affinity chromatography.

Immunogen: Highly pure (>98%) recombinant hSCF (anti-hSCF).

Application Details

Application Notes: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofhSCF (10 ng/mL), a concentration of 8 - 12 ng/mL of this antibody is required. ELISA: To detect hSCF by direct ELISA (using 100 l/well antibody solution) a concentrationof at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, inconjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/wellof recombinant hSCF. Western Blot: To detect hSCF by Western Blot analysis this antibody can be used at aconcentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hSCF is 1.5 - 3.0 ng/lane, under either reducing ornon-reducing conditions. Immunohistochemistry: 1 μg/mL is the recommended starting dilution for frozen andparaffin sections. For paraffin sections antigen retrieval techniques may be necesserary toimprove staining.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing. Centrifuge vial prior to opening!

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Target Details for KIT Ligand

Target: KIT Ligand (KITLG)

Alternative Name: KITLG / SCF

Background: Synonyms: KITL, Kit ligand, MGF, Mast cell growth factor, Stem cell factor, c-Kit ligand

Gene ID: 4254

NCBI Accession: NP_000890

UniProt: P21583

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway