CCL17 antibody

Catalog No. ABIN116237

The Rabbit Polyclonal anti-CCL17 antibody has been validated for WB, EIA and Func. It is suitable to detect CCL17 in samples from Human.

Quick Overview for CCL17 antibody (ABIN116237)

Target: CCL17 (Chemokine (C-C Motif) Ligand 17 (CCL17))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CCL17 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA), Functional Studies (Func)

Product Details anti-CCL17 Antibody

Specificity: Thism antibody reacts with TARC (CCL17).

Purification: Affinity chromatography.

Immunogen: Highly pure (>98%) recombinant hTARC.

Application Details

Application Notes: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofhTARC (100 ng/mL), a concentration of 20-30 μg/mL of this antibody is required. ELISA: (Indirect): To detect hTARC by indirect ELISA (using 100 μL/well antibody solution) aconcentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with compatible secondary reagents, allows the detection of atleast 0.2-0.4 ng/well of recombinant hTARC. (Sandwich): To detect hTARC by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with Biotinylated Anti-Human TARC (PP1069B) as a detectionantibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hTARC. Western Blot: To detect hTARC by Western Blot analysis this antibody can be used at aconcentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hTARC is 1.5-3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/mL

Buffer: PBS, pH 7.2 without preservatives.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Target Details for CCL17

Target: CCL17 (Chemokine (C-C Motif) Ligand 17 (CCL17))

Alternative Name: TARC / CCL17

Background: Synonyms: C-C motif chemokine 17, CC chemokine TARC, SCYA17, Small-inducible cytokine A17, Thymus and activation-regulated chemokine

Gene ID: 6361

NCBI Accession: NP_002978

UniProt: Q92583