LTA antibody (Biotin)

Catalog No. ABIN116245

This Rabbit Polyclonal antibody specifically detects LTA in WB and EIA. It exhibits reactivity toward .

Quick Overview for LTA antibody (Biotin) (ABIN116245)

Target: LTA (Lymphotoxin-alpha (LTA))

Reactivity: Please inquire

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LTA antibody is conjugated to Biotin

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-LTA Antibody (Biotin)

Specificity: This antibody reacts with Tumor Necrosis Factor beta.

Purification: Affinity chromatography.

Immunogen: Highly pure (>98%) recombinant hTNF-beta.

Application Details

Application Notes: ELISA: (Direct): To detect hTNF-β by direct ELISA (using 100 μL/well antibody solution) aconcentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylated polyclonalantibody, in conjunction with compatible secondary reagents, allows the detection of atleast 0.2-0.4 ng/well of recombinant hTNF-β. (Sandwich): To detect hTNF-β by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylated polyclonalantibody, in conjunction with Polyclonal Anti-Human TNF-β (PP1072P1/PP1072P2) as acapture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hTNF-β. Western Blot: To detect hTNF-β by Western Blot analysis this antibody can be used at aconcentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hTNF-β is 1.5- 3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Target Details for LTA

Target: LTA (Lymphotoxin-alpha (LTA))

Alternative Name: TNF-beta / Lymphotoxin-alpha

Target Type: Chemical

Background: Synonyms: LT-alpha, LTA, TNFB, TNFSF1, Tumor necrosis factor ligand superfamily member 1

Gene ID: 4049

NCBI Accession: NP_000586

UniProt: P01374

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process