LTA antibody

Catalog No. ABIN116247

This anti-LTA antibody is a Rabbit Polyclonal antibody detecting LTA in WB, EIA and Func. Suitable for Human.

Quick Overview for LTA antibody (ABIN116247)

Target: LTA (Lymphotoxin-alpha (LTA))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LTA antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA), Functional Studies (Func)

Product Details anti-LTA Antibody

Specificity: This antibody reacts with Tumor Necrosis Factor beta.

Purification: Affinity chromatography.

Immunogen: Highly pure (>98%) recombinant hTNF-beta (human Tumor Necrosis Factor-beta).

Application Details

Application Notes: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofhTNF-beta (0.12 ng/mL), a concentration of 0.002-0.004 μg/mL of this antibody is required. ELISA: (Indirect): To detect hTNF-β by indirect ELISA (using 100 μL/well antibody solution) aconcentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with compatible secondary reagents, allows the detection of atleast 0.2 - 0.4 ng/well of recombinant hTNF-β. (Sandwich): To detect hTNF-β by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with Biotinylated Anti-Human TNF-β (PP1072B) as a detectionantibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hTNF-β. Western Blot: To detect hTNF-β by Western Blot analysis this antibody can be used at aconcentration of 0.1- 0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hTNF-β is 1.5-3.0 ng/lane, under either reducingnon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/mL

Buffer: PBS, pH 7.2 without preservatives.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Target Details for LTA

Target: LTA (Lymphotoxin-alpha (LTA))

Alternative Name: TNF-beta / Lymphotoxin-alpha

Target Type: Chemical

Background: Synonyms: LT-alpha, LTA, TNFB, TNFSF1, Tumor necrosis factor ligand superfamily member 1

Gene ID: 4049

NCBI Accession: NP_000586

UniProt: P01374

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process