Thrombopoietin antibody (Biotin)

Catalog No. ABIN116253

The Biotin-conjugated Goat Polyclonal anti-Thrombopoietin antibody (ABIN116253) specifically detects Thrombopoietin in WB and EIA. The antibody is reactive with Human samples.

Quick Overview for Thrombopoietin antibody (Biotin) (ABIN116253)

Target: Thrombopoietin (THPO)

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This Thrombopoietin antibody is conjugated to Biotin

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-Thrombopoietin Antibody (Biotin)

Specificity: Reacts with Thrombopoietin.

Purification: Affinity chromatography.

Immunogen: Highly pure (>98%) recombinant hTPO.

Application Details

Application Notes: ELISA: To detect hTPO by direct ELISA (using 100 l/well antibody solution) this antibodycan be used at a concentration of 0.15 - 0.30 μg/mL. Used in conjunction with compatiblesecondary reagents, allows the detection of at least 0.2 ng/well of recombinant hTPO. Western Blot: To detect hTPO by Western Blot analysis this antibody can be used at aconcentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hTPO is 1.5 - 3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Target Details for Thrombopoietin

Target: Thrombopoietin (THPO)

Alternative Name: Thrombopoietin

Background: Synonyms: C-mpl ligand, MGDF, Megakaryocyte colony-stimulating factor, Megakaryocyte growth and development factor, Myeloproliferative leukemia virus oncogene ligand, THPO

Gene ID: 7066

NCBI Accession: NP_000451

UniProt: P40225

Pathways: JAK-STAT Signaling, Hormone Activity