CXCL10 antibody

Catalog No. ABIN116271

The Rabbit Polyclonal anti-CXCL10 antibody (ABIN116271) specifically detects CXCL10 in WB, EIA and Func. The antibody is reactive with Mouse samples.

Quick Overview for CXCL10 antibody (ABIN116271)

Target: CXCL10 (Chemokine (C-X-C Motif) Ligand 10 (CXCL10))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CXCL10 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA), Functional Studies (Func)

Product Details anti-CXCL10 Antibody

Specificity: Reacts with Interferon-gamma Inducible Protein-10

Purification: Affinity Chromatography using an immobilizedd Murine IP-10 Matrix

Immunogen: Highly pure (>98%) E.coli derived recombinant Mouse IP-10.

Application Details

Application Notes: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofMouse IP-10 (100 ng/mL), a concentration of 10.0 μg/mL of this antibody is required. Sandwich ELISA: To detect Mouse IP-10 by Sandwich ELISA (using 100 ml/well antibodysolution), a concentration of 0.5-2.0 μg/mL of this antibody is required. This Biotinylatedantibody, in conjuction with anti Mouse IP10 antibody (cat. PP1083B) as a Detectionantibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Mouse IP-10. Western Blot: To detect Mouse IP-10 by Western Blot analysis this antibody can be used ata concentration of 0.1 -0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant Mouse IP-10 is 1.5-3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Target Details for CXCL10

Target: CXCL10 (Chemokine (C-X-C Motif) Ligand 10 (CXCL10))

Alternative Name: CXCL10 / IP-10

Background: Interferon-gamma-inducible 10 kD protein (IP-10), is a CXC chemokine with chemoattractant properties for CD4-positive T cells and inhibits early normal and leukemic hemopoietic progenitor proliferation. IP-10 is produced by a wide variety of cell types ranging from neutrophils and monocytes to hepatocytes, endothelial cells and keratinocytes. The cytokine is reported to be involved in a scala of inflammatory pathologies such as HIV encephalitis, cutaneous T cell lymphoma, chronic hepatitis and acute anterior uveitis. Various observations strongly suggest a role for the CXC chemokines IL-8 and IP-10 in the regulation of angiogenic activity in cancer and in idiopathic pulmonary fibrosis.Synonyms: 10 kDa interferon-gamma-induced protein, C-X-C motif chemokine 10, Gamma-IP10, INP10, SCYB10, Small-inducible cytokine B10

Gene ID: 15945

NCBI Accession: NP_067249

UniProt: P17515