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CCL23 antibody (Biotin)

This Rabbit Polyclonal antibody specifically detects CCL23 in WB and EIA. It exhibits reactivity toward Human.
Catalog No. ABIN116287

Quick Overview for CCL23 antibody (Biotin) (ABIN116287)

Target

See all CCL23 Antibodies
CCL23 (Chemokine (C-C Motif) Ligand 23 (CCL23))

Reactivity

  • 36
  • 1
Human

Host

  • 28
  • 8
Rabbit

Clonality

  • 30
  • 6
Polyclonal

Conjugate

  • 26
  • 4
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This CCL23 antibody is conjugated to Biotin

Application

  • 25
  • 16
  • 12
  • 9
  • 9
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
Western Blotting (WB), Enzyme Immunoassay (EIA)
  • Specificity

    The antibody reacts with Macrophage Inflammatory Protein-3.

    Purification

    Affinity Chromatography

    Immunogen

    Highly pure (>98%) E.coli derived recombinant Human MIP-3.
  • Application Notes

    ELISA: To detect hMIP-3 by direct ELISA (using 100 l/well antibody solution) this antibody can beused at a concentration of 0.25 - 1.0 μg/mL. Used in conjunction with compatible secondaryreagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hMIP-3. To detect hMIP-3 by sandwich ELISA (using 100 l/well antibody solution) a concentrationof 0.25 - 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, inconjuction with anti-human MIP-3 (cat-no. PP1089P1/2) as a capture antibody, allows thedetection of at least 0.2 - 0.4 ng/well of recombinant human MIP-3. Western Blot: To detect hMIP-3 by Western Blot analysis this antibody can be used at a concentration of0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detectionlimit for recombinant hMIP-3 is 1.5 - 3.0 ng/lane, under either reducing or non-reducingconditions.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    Restrictions

    For Research Use only
  • Reconstitution

    Centrifuge the vial prior to opening. Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.

    Buffer

    PBS, pH 7.2

    Handling Advice

    Avoid repeated freezing and thawing.

    Storage

    4 °C/-20 °C

    Storage Comment

    The lyophilized antibody is stable at -20 °C for one year from date of despatch. Reconstituted antibody is stable for 2 weeks at 2-8 °C and for six months when stored in working aliquots at -20 °C.

    Expiry Date

    12 months
  • Target

    CCL23 (Chemokine (C-C Motif) Ligand 23 (CCL23))

    Alternative Name

    MIP3 / CCL23

    Background

    Macrophage Inflammatory Protein 3 (MIP3) is chemotactic for monocytes, resting T-lymphocytes, and neutrophils. It inhibits proliferation of myeloid progenitor cells and is highly expressed in adult lung, liver, skeletal muscle and pancreas. There are moderate levels of MIP3 in fetal liver, adult bone marrow and placenta. MIP3 belongs to the intercrine beta family (small cytokine c-c) (chemokine c-c).Synonyms: C-C motif chemokine 23, CCL-23, CK-beta-8, CKB-8, MIP-3, MPIF1, Macrophage inflammatory protein 3, Myeloid progenitor inhibitory factor 1, SCYA23, Small-inducible cytokine A23

    Gene ID

    6368

    NCBI Accession

    NP_005055

    UniProt

    P55773
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